2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Enzyme gawo lapansi: ONPG imagwiritsidwa ntchito ngati gawo lapansi la beta-galactosidase, puloteni yomwe imatulutsa ONPG kuti ipange gulu lachikaso (o-nitrophenol) lomwe limatha kuzindikirika mosavuta.Kuchita kwa enzymatic kumeneku kungagwiritsidwe ntchito kuyeza ntchito ya beta-galactosidase, kupanga ONPG chida chofunikira pamaphunziro a enzymology.

Mayeso a biology ya mamolekyulu: ONPG imagwiritsidwa ntchito ngati gawo laling'ono pazoyesa zosiyanasiyana zamamolekyulu, makamaka mu zoyeserera zamtundu wa beta-galactosidase reporter.M'mayesero awa, gawo laling'ono lochokera ku ONPG limagwiritsidwa ntchito kuyeza zochita za mtolankhani, zomwe nthawi zambiri zimayendetsedwa ndi gulu linalake lokonda chidwi.Ntchito ya beta-galactosidase, yowonetsedwa ndi kusintha kwa utoto komwe kumapangidwa pa ONPG hydrolysis, imapereka chidziwitso chofunikira pazantchito zolimbikitsa.

Kusanthula kwa mawu a gene: ONPG imagwiritsidwanso ntchito pakuwunika mawu amtundu.Polumikiza kutsatizana kwa chidwi ndi jini ya beta-galactosidase, ofufuza amatha kuyeza zochitika za beta-galactosidase pogwiritsa ntchito ONPG ngati gawo lapansi.Mulingo wa ntchito ya beta-galactosidase ukuwonetsa mphamvu ndi zochita za olimbikitsa, kulola kuwunika kwa milingo ya jini.

Ntchito zowunikira: ONPG itha kugwiritsidwa ntchito pozindikira matenda, makamaka pozindikira tizilombo toyambitsa matenda.Mabakiteriya osiyanasiyana, monga Escherichia coli ndi mitundu ina ya Shigella ndi Salmonella, amapanga beta-galactosidase yomwe imatha kuthyola ONPG.Izi hydrolysis anachita umatulutsa lowoneka mtundu kusintha, amene angagwiritsidwe ntchito kudziwa kukhalapo kwa mabakiteriya mu zitsanzo zachipatala.