2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Kapangidwe ka Enzyme: ONPG nthawi zambiri imagwiritsidwa ntchito ngati kapangidwe ka beta-galactosidase, enzyme yomwe imathira ONPG kuti ipange mankhwala achikasu (o-nitrophenol) omwe amatha kuzindikirika mosavuta pogwiritsa ntchito spectrophotometric. Kapangidwe ka enzymatic aka kangagwiritsidwe ntchito kuyeza ntchito ya beta-galactosidase, zomwe zimapangitsa ONPG kukhala chida chofunikira kwambiri pamaphunziro a enzymology.

Kuyesa kwa biology ya molekyulu: ONPG imagwiritsidwa ntchito ngati gawo loyambira mu mayeso osiyanasiyana a biology ya molekyulu, makamaka mu mayeso a jini yowunikira ya beta-galactosidase. Mu mayeso awa, gawo loyambira la ONPG limagwiritsidwa ntchito kuyeza ntchito ya jini yowunikira, yomwe nthawi zambiri imayendetsedwa ndi mndandanda winawake wa chidwi cha promoter. Ntchito ya beta-galactosidase, yomwe imawonetsedwa ndi kusintha kwa mtundu komwe kumachitika pa hydrolysis ya ONPG, imapereka chidziwitso chofunikira chokhudza ntchito yowunikira.

Kusanthula momwe majini amagwirira ntchito: ONPG imagwiritsidwanso ntchito posanthula momwe majini amagwirira ntchito. Mwa kulumikiza mndandanda wa zotsatsa zomwe zimakhudzidwa ndi jini ya beta-galactosidase, ofufuza amatha kuyeza momwe beta-galactosidase imagwirira ntchito pogwiritsa ntchito ONPG ngati gawo lapansi. Mlingo wa momwe beta-galactosidase imagwirira ntchito umasonyeza mphamvu ndi ntchito ya wotsatsa, zomwe zimathandiza kuwunika kuchuluka kwa momwe majini amagwirira ntchito.

Kugwiritsa ntchito pozindikira matenda: ONPG ingagwiritsidwe ntchito pozindikira matenda, makamaka pozindikira tizilombo toyambitsa matenda. Mabakiteriya osiyanasiyana, monga Escherichia coli ndi mitundu ina ya Shigella ndi Salmonella, amapanga beta-galactosidase yomwe imatha kugawa ONPG. Kuphatikizika kwa hydrolysis kumeneku kumapangitsa kusintha kwa mtundu, komwe kungagwiritsidwe ntchito kudziwa kupezeka kwa mabakiteriyawa m'zitsanzo zachipatala.