5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ndi gulu lomwe limagwiritsidwa ntchito m'maphunziro osiyanasiyana a biochemical, makamaka pozindikira ndikuwona zochitika za enzyme.Ndi gawo lapansi lomwe limatha kupangidwa ndi hydrolyzed ndi michere inayake, zomwe zimapangitsa kutulutsa kwamtundu wamtundu kapena fulorosenti.

Pagululi nthawi zambiri amagwiritsidwa ntchito poyesa kudziwa kukhalapo ndi zochita za michere monga beta-galactosidase ndi beta-glucuronidase.Ma enzymes awa amadula magulu a acetyl ndi glucosaminide kuchokera ku gawo lapansi, zomwe zimapangitsa kupanga chromophore ya buluu kapena yobiriwira.

Mapangidwe apadera a 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide amalola kuti azindikire mosavuta ndi kuchuluka kwa ntchito ya enzyme.Kugwiritsiridwa ntchito kwake mu njira zosiyanasiyana zoyesera, kuphatikizapo histochemistry, immunohistochemistry, ndi ma cell-based assays, zathandizira kumvetsetsa bwino ntchito za enzyme.

Konzani kapena Funsani Mtengo

Tsatanetsatane wa Zamalonda

Zogulitsa Tags

Kugwiritsa Ntchito ndi Zotsatira

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) ndi gawo lapansi la chromogenic lomwe limagwiritsidwa ntchito pozindikira ntchito ya beta-glucuronidase (GUS).GUS ndi puloteni yomwe imapezeka m'zamoyo zosiyanasiyana, kuphatikizapo mabakiteriya, zomera, ndi zinyama.X-Gluc imagwiritsidwa ntchito pafupipafupi pakuyesa atolankhani a GUS komanso kafukufuku wamamolekyulu a biology.

Kugwiritsa ntchito kwakukulu kwa X-Gluc kuli mu njira zodetsa za histochemical, komwe kumatha kuwona momwe ma enzyme a GUS amawonekera.Gawo laling'onoli limatha kulowa m'maselo ndipo limapangidwa ndi hydrolyzed ndi GUS, zomwe zimapangitsa kuti pakhale buluu kapena chinthu chosasungunuka.Kudetsa kwa buluu kumeneku kumathandizira ofufuza kuzindikira ndikuyika zochitika za GUS m'maselo, minofu, ndi zamoyo zonse.

X-Gluc itha kugwiritsidwanso ntchito poyesa kuchuluka kwake kuyesa ntchito ya GUS enzyme.Kuchuluka kwa mtundu wa buluu kapena kuchuluka kwa zinthu zomwe zimapangidwa zimatha kulumikizidwa ndi kuchuluka kwa mawu a GUS kapena ntchito yake ya enzymatic.

Kuphatikiza apo, X-Gluc yagwiritsidwa ntchito pofufuza zamtundu wa zomera kuti iphunzire za jini, zochitika zolimbikitsa, ndi kusintha kwa zomera.Amagwiritsidwanso ntchito m'mabakiteriya opangira ma cloning ndi kuzindikira mapuloteni a GUS fusion.

Kulongedza katundu:

Zina Zowonjezera:

Kupanga	C16H18BrClN2O6
Kuyesa	99%
Maonekedwe	White ufa
CAS No.	4264-82-8
Kulongedza	Zing'onozing'ono ndi zambiri
Shelf Life	zaka 2
Kusungirako	Sungani pamalo ozizira ndi owuma
Chitsimikizo	ISO.