HEPES CAS: 7365-45-9 Mtengo Wopanga

pH buffering: HEPES imagwiritsidwa ntchito kwambiri ngati chotchinga chosungira kuti pH ikhale yokhazikika pama media amtundu wama cell komanso kuyesa kwachilengedwe.Zimagwira ntchito bwino pamtundu wa pH wa 6.8 mpaka 8.2, ndikupangitsa kuti ikhale yoyenera kukhalabe ndi mikhalidwe yabwino kwambiri yazachilengedwe.

Chikhalidwe cha ma cell: HEPES nthawi zambiri imawonjezeredwa ku media media kuti akhazikike mulingo wa pH, ndikupereka malo abwino oti ma cell akule komanso kuti ma cell azikhala bwino.Zimathandizira kupewa kusinthasintha kwa pH komwe kungasokoneze machitidwe a cell ndi zotsatira zoyesera.

Kuyeza kwa ma enzyme: HEPES imagwiritsidwa ntchito nthawi zambiri ngati chotchingira pamachitidwe a enzymatic chifukwa chakutha kwake kusunga pH yeniyeni.Zimathandizira kukhalabe ndi magwiridwe antchito abwino a enzymatic ndi kukhazikika, potero kumakulitsa kulondola komanso kudalirika kwa kuyesa kwa ma enzyme.

Maphunziro a mapuloteni: HEPES imagwiritsidwa ntchito poyesera zosiyanasiyana zokhudzana ndi mapuloteni, kuphatikizapo kuyeretsa mapuloteni, crystallization ya mapuloteni, ndi kusanthula kapangidwe ka mapuloteni.Zimathandizira kukhazikika kwa pH ndikusunga mapuloteni oyenera komanso kukhazikika panthawi yoyeserera.

Electrophoresis: HEPES imapeza kugwiritsa ntchito njira za gel electrophoresis, monga SDS-PAGE ndi agarose gel electrophoresis.Imagwiritsidwa ntchito ngati chotchinga chotchinga mu buffer yothamanga, kupereka malo okhazikika a pH olekanitsa ndi kusanthula ma biomolecules.

Mapangidwe a mankhwala: HEPES imagwiritsidwa ntchito popanga mankhwala ndi biotechnological mankhwala.Zimathandizira kukhalabe okhazikika komanso kukhulupirika kwa zosakaniza zogwira ntchito, kuonetsetsa kuti zikugwira ntchito bwino komanso nthawi ya alumali.