IPTG CAS:367-93-1 Mtengo Wopanga
Isopropyl β-D-1-thiogalactopyranoside (IPTG) ndi analogue yopangidwa ndi lactose yomwe imagwiritsidwa ntchito kwambiri pofufuza kafukufuku wa mamolekyulu a biology ndi biotechnology application.IPTG imagwiritsidwa ntchito kulimbikitsa kufotokozera kwa majini m'makina a bakiteriya, komwe kumakhala ngati choyambitsa mamolekyulu kuti ayambitse kusindikiza kwa jini.
Mukawonjezeredwa ku sing'anga ya kukula, IPTG imatengedwa ndi mabakiteriya ndipo imatha kumangirira ku mapuloteni a lac repressor, kuti asatseke ntchito ya lac operon.Lac operon ndi gulu la majini omwe amakhudzidwa ndi lactose metabolism, ndipo mapuloteni opondereza akachotsedwa, majini amawonekera.
IPTG imagwiritsidwa ntchito nthawi zambiri molumikizana ndi lacUV5 mutant promotioner, yomwe ndi mtundu waposachedwa wa olimbikitsa lac.Pophatikiza kulowetsedwa kwa IPTG ndi wolimbikitsa wosinthika uyu, ofufuza atha kukwaniritsa mawonekedwe apamwamba amtundu.Izi zimathandiza kupanga mapuloteni ochuluka kuti ayeretsedwe kapena ntchito zina zapansi.
Kuphatikiza pa mafotokozedwe a jini, IPTG imagwiritsidwanso ntchito pafupipafupi pakuyesa buluu / koyera.Mwanjira imeneyi, jini ya lacZ nthawi zambiri imaphatikizidwa ku jini yosangalatsa, ndipo mabakiteriya omwe amafotokoza bwino jini ya fusion iyi amatulutsa enzyme yogwira ya β-galactosidase.IPTG ikawonjezeredwa pamodzi ndi gawo lapansi la chromogenic monga X-gal, mabakiteriya omwe amawonetsa jini ya fusion amasanduka buluu chifukwa cha ntchito ya β-galactosidase.Izi zimalola kuzindikiritsa ndi kusankha mitundu yophatikizanso yomwe yaphatikiza bwino jini yachidwi.
Kupititsa patsogolo mawu a jini: IPTG imagwiritsidwa ntchito nthawi zambiri kukopa kufotokozera kwa majini omwe amatsata m'mabakiteriya.Imatsanzira lactose yachilengedwe yotulutsa lactose ndikumangirira ku mapuloteni a lac repressor, kuwaletsa kutsekereza lac operon.Izi zimalola kulembedwa ndi kufotokoza kwa majini omwe mukufuna.
Kufotokozera kwa mapuloteni ndi kuyeretsa: Kulowetsa kwa IPTG nthawi zambiri kumagwiritsidwa ntchito popanga mapuloteni ambiri ophatikizananso pazinthu zosiyanasiyana, monga maphunziro a biochemical, kupanga mankhwala, kapena kusanthula kamangidwe.Pogwiritsa ntchito ma vector oyenerera komanso kulowetsa kwa IPTG, ofufuza amatha kukwaniritsa kuchuluka kwa mapuloteni omwe amapangidwa ndi mabakiteriya.
Kuwunika kwa buluu/kuyera: IPTG imagwiritsidwa ntchito pafupipafupi kuphatikiza jini ya lacZ ndi gawo lapansi la chromogenic, monga X-gal, poyesa kuyesa kwa buluu/kuyera.Jini la lacZ nthawi zambiri limasakanikirana ndi jini yosangalatsa, ndipo mabakiteriya omwe amafotokozera bwino jini ya fusion iyi amatulutsa enzyme yogwira ya β-galactosidase.IPTG ndi gawo lapansi la chromogenic likawonjezedwa, mitundu yophatikizanso yomwe ikuwonetsa jini ya fusion imasanduka buluu, zomwe zimapangitsa kuti zizindikirike ndikusankha mosavuta.
Kusanthula kwa gene regulation: IPTG induction imagwiritsidwa ntchito kwambiri pofufuza kuti aphunzire kuwongolera kwa majini ndi ma operens, makamaka lac operon.Mwa kuwongolera kuchuluka kwa IPTG ndikuwunika mawonekedwe a zigawo za lac operon, ofufuza amatha kufufuza njira zoyendetsera ma jini ndi udindo wa zinthu zosiyanasiyana kapena masinthidwe.
Mawonekedwe a gene: IPTG ndi gawo lofunikira kwambiri pamachitidwe angapo amtundu wa jini, monga machitidwe olimbikitsa a T7.M'makinawa, wolimbikitsa lac nthawi zambiri amagwiritsidwa ntchito poyendetsa mawu a T7 RNA polymerase, omwe, nawonso, amalemba ma jini omwe akuwongolera motsogozedwa ndi zotsatizana za T7.IPTG imagwiritsidwa ntchito kulimbikitsa mafotokozedwe a T7 RNA polymerase, zomwe zimapangitsa kuti mawu a jini ayambike.
| Kupanga | Chithunzi cha C9H18O5S |
| Kuyesa | 99% |
| Maonekedwe | White ufa |
| CAS No. | 367-93-1 |
| Kulongedza | Zing'onozing'ono ndi zambiri |
| Shelf Life | zaka 2 |
| Kusungirako | Sungani pamalo ozizira ndi owuma |
| Chitsimikizo | ISO. |
