PIPES CAS: 5625-37-6 Mtengo Wopanga

PIPES (piperazine-1,4-bisethanesulfonic acid) ndi zwitterionic buffering compound yomwe imagwiritsidwa ntchito makamaka pakufufuza kwachilengedwe ndi zamankhwala.Ili ndi zinthu zingapo zofunika ndi ntchito, kuphatikiza:

pH buffering agent: PIPES ndi chitetezo chothandiza chomwe chimathandiza kusunga pH yokhazikika pamayesero osiyanasiyana achilengedwe.Amagwiritsidwa ntchito kwambiri pama media amtundu wama cell, kuyesa kwa ma enzyme, komanso kugwiritsa ntchito ma cell biology.

Kuthekera kwakukulu kotchinga: PIPES ili ndi mphamvu yabwino yotchinga mkati mwa pH ya 6.1 mpaka 7.5, kupangitsa kuti ikhale yoyenera kusunga pH yokhazikika pamakina osiyanasiyana achilengedwe.

Kuyanjana kochepa ndi ma biomolecules: PIPES imadziwika chifukwa chosokoneza pang'ono ndi njira zama biochemical komanso kumangirira pang'ono kwa mapuloteni ndi michere, zomwe zimapangitsa kukhala koyenera kusunga umphumphu ndi ntchito za ma biomolecules.

Oyenera kuyesa kutengera kutentha: PIPES imatha kusunga mawonekedwe ake obisala pa kutentha kwakukulu, kuphatikiza kutentha kwa thupi ndi kokwezeka.Izi zimapangitsa kuti ikhale yoyenera pazoyeserera zomwe zimafuna kukhazikika komanso kulondola pamikhalidwe yosiyanasiyana ya kutentha.

Electrophoresis applications: PIPES amagwiritsidwa ntchito ngati chotchingira mu njira za gel electrophoresis, monga RNA kapena DNA agarose gel electrophoresis, chifukwa cha kutsika kwake kwa UV komanso kutulutsa kwake kwakukulu.

Kupanga mankhwala: PIPES imagwiritsidwanso ntchito popanga mankhwala monga chosungira, kupereka bata ndi kusunga pH yoyenera kuti mankhwala agwire bwino.