X-GAL CAS:7240-90-6 Mtengo Wopanga
Kusintha kwa Mtundu: X-Gal nthawi zambiri imakhala yopanda utoto koma, ikapangidwa ndi β-galactosidase, imasanduka yabuluu.Kusintha kwa mtundu uku kumathandizira kuzindikira komanso kuwerengetsa kwa ntchito ya β-galactosidase.
Kuzindikira kwa LacZ Gene: X-Gal imagwiritsidwa ntchito kuzindikira maselo kapena ma genetic omwe amawonetsa jini ya lacZ.LacZ imagwiritsidwa ntchito nthawi zambiri ngati jini ya mtolankhani mu biology ya mamolekyulu kuti awone momwe ma jini amatchulidwira kapena zochitika zolimbikitsa maphunziro.
Kuwunika kwa Colony: X-Gal imagwiritsidwa ntchito poyesa kuyesa kwa mabakiteriya.Mabakiteriya owonetsa LacZ amawoneka a buluu akakula pa agar omwe ali ndi X-Gal, zomwe zimathandiza kuzindikira ndikusankha madera omwe ali ndi lacZ-positive.
Gene Fusion Analysis: X-Gal imagwiritsidwanso ntchito poyesa gene fusion.Pamene jini yowunikira imalumikizidwa ndi jini ya lacZ, madontho a X-Gal amatha kuwulula mawonekedwe a mapuloteni ophatikizika mkati mwa selo kapena minofu.
Kukhazikika kwa Mapuloteni: Madontho a X-Gal atha kugwiritsidwa ntchito kuti afufuze momwe mapuloteni amtundu wa subcellular amapezeka.Pophatikiza puloteni yosangalatsa ku jini ya lacZ, ntchito ya β-galactosidase imatha kuwonetsa komwe mapuloteni amakhala mkati mwa selo.
X-Gal Analogues: Mitundu yosinthidwa ya X-Gal, monga Bluo-Gal kapena Red-Gal, yapangidwa kuti ilole njira zina zopangira mitundu.Ma analoguewa amathandiza kusiyanitsa pakati pa maselo a lacZ-positive ndi lacZ-negative kapena minyewa pogwiritsa ntchito mitundu yosiyanasiyana.
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Kupanga | C14H15BrClNO6 |
Kuyesa | 99% |
Maonekedwe | White ufa |
CAS No. | 7240-90-6 |
Kulongedza | Zing'onozing'ono ndi zambiri |
Shelf Life | zaka 2 |
Kusungirako | Sungani pamalo ozizira ndi owuma |
Chitsimikizo | ISO. |