3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

Ndi chipangizo choyezera kuwala chomwe chili ndi gulu la nitrobenzoxadiazole (NBD), lomwe limasonyeza mphamvu zamphamvu za kuwala. Gulu la aldehyde limalola kulemba ndi kuzindikira ma biomolecule monga mapuloteni, nucleic acid, ndi lipids.

NBD-aldehyde nthawi zambiri imagwiritsidwa ntchito ngati utoto wochitapo kanthu poyesa kuzindikira ndi kuyeza momwe mapuloteni kapena ma peptide amagwirira ntchito, kusintha kwa mapuloteni, ndi kuyanjana kwa mapuloteni ndi mapuloteni. Imatha kulemba magulu a amino aulere pamapuloteni kapena ma peptide, ndikupanga ma covalent bonds okhazikika. Izi zimapangitsa kuti ikhale yothandiza pa ntchito monga immunocytochemistry, immunohistochemistry, ndi western blotting.

Kuwala kwa NBD-aldehyde kumatha kusinthidwa ndi kuwala kwa ultraviolet kapena buluu, ndipo kumatulutsa kuwala kobiriwira. Kapangidwe kameneka kamathandiza kuwona ndi kuwerengera ma biomolecules olembedwa pogwiritsa ntchito njira za fluorescence microscopy kapena spectroscopy.