3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

3-Nitrophenyl-beta-D-galactopyranoside (ONPG) ndi chinthu chomwe chimagwiritsidwa ntchito kwambiri poyesa ma enzyme kuti chizindikire ndikuyesa ntchito ya beta-galactosidase. Pamene beta-galactosidase ilipo ndipo ikugwira ntchito, imathira ONPG ndi madzi, kutulutsa chinthu chachikasu chotchedwa 3-nitrophenol. Mphamvu ya mtundu wachikasu wopangidwa imatha kuyezedwa ndi spectrophotometric, zomwe zimathandiza kuwerengera kuchuluka kwa ntchito ya beta-galactosidase. ONPG imagwiritsidwa ntchito nthawi zambiri mu kafukufuku wa ma molecular biology ndi microbiology, komanso pofufuza zachipatala, kuti iphunzire momwe majini amagwirira ntchito, ntchito ya mapuloteni, kuzindikira mabakiteriya, komanso momwe maselo amagwirira ntchito.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kuzindikira ntchito ya beta-galactosidase: ONPG nthawi zambiri imagwiritsidwa ntchito kudziwa kupezeka ndi ntchito ya beta-galactosidase m'zitsanzo zosiyanasiyana zamoyo, monga mabakiteriya kapena ma cell lysates. Kupanga kwa o-nitrophenol, komwe kuli ndi mtundu wachikasu, kumatha kuyezedwa mosavuta pogwiritsa ntchito spectrophotometer.

Maphunziro a majini: ONPG imagwiritsidwa ntchito kwambiri mu kafukufuku wa sayansi ya mamolekyulu kuti iphunzire za majini. Mwa kuphatikiza cholimbikitsira jini yosangalatsa ndi jini yomwe imapanga beta-galactosidase, ofufuza amatha kuyeza ntchito ya cholimbikitsira ichi powonjezera ONPG ndikuwerengera kupanga kwa o-nitrophenol komwe kumachitika. Njira iyi, yomwe imadziwika kuti beta-galactosidase reporter assay, imapereka chidziwitso chokhudza ntchito yolemba ya jini.

Kuzindikira mabakiteriya: Mabakiteriya ena amapanga beta-galactosidase, pomwe ena sachita. ONPG ingagwiritsidwe ntchito limodzi ndi mayeso ena a biochemical kuti adziwe mitundu ya mabakiteriya kutengera mphamvu yawo yothira ONPG. Njirayi imagwiritsidwa ntchito kwambiri m'ma laboratories azachipatala komanso m'ma laboratories a microbiology.

Kufufuza zinthu zoletsa ma enzyme kapena zoyambitsa: ONPG ingagwiritsidwe ntchito kufufuza zinthu zomwe zimasinthasintha ntchito ya beta-galactosidase. Poyesa ntchito ya ma enzyme pamaso pa zinthu zosiyanasiyana, ofufuza amatha kuzindikira zinthu zomwe zingalepheretse kapena zoyambitsa zomwe zingafufuzidwe kwambiri kuti adziwe momwe zimathandizira.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C12H15NO8
Kuyesa	99%
Maonekedwe	Choyeraufa
Nambala ya CAS	3150-25-2
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.