3,3 ,5,5 -Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

3,3', 5,5'-Tetramethylbenzidine dihydrochloride (TMB) ndi gawo logwiritsidwa ntchito kwambiri mu mayeso a enzyme-linked immunosorbent assays (ELISAs) ndi mayeso ena a biochemical. Pamene TMB imasungunuka ndi enzyme horseradish peroxidase (HRP) pamaso pa hydrogen peroxide, imapanga mtundu wabuluu. Kusintha kwa mtundu kumeneku kumagwiritsidwa ntchito kuzindikira kupezeka kwa ma antibodies olembedwa ndi HRP kapena mapuloteni ena, nthawi zambiri kusonyeza kupezeka kwa antigen inayake mu chitsanzo. Mtundu wabuluu womwe umapangidwa ndi zomwe zimachitika nthawi zambiri umayesedwa pogwiritsa ntchito spectrophotometer, yomwe imalola kuyeza mphamvu ya kusintha kwa mtundu ndipo imapereka njira yolumikizira mphamvu ya mtundu ndi kuchuluka kwa molekyulu yomwe ilipo mu chitsanzo. TMB ili ndi ntchito zambiri mu mayeso ozindikira, kafukufuku, ndi chitukuko cha mankhwala, makamaka pakupeza ma antibodies, mahomoni, ma cytokine, ndi ma biomolecule ena. Kuzindikira kwake, kukhazikika kwake, komanso kusavuta kugwiritsa ntchito kumapangitsa kuti ikhale chida chofunikira m'malo osiyanasiyana a labotale kuti izindikire ndi kuwerengera mamolekyu enaake.