3,3′,5,5′-Tetramethylbenzidine sulfate CAS:54827-18-8

ELISA: Imagwiritsidwa ntchito kwambiri ngati gawo la horseradish peroxidase (HRP) mu mayeso a ELISA. Pakakhala molekyulu yofunidwa, monga antigen kapena antibody, enzyme HRP imayambitsa okosijeni wa 3,3', 5,5'-Tetramethylbenzidine sulfate, zomwe zimapangitsa kuti pakhale chinthu chabuluu. Mphamvu ya utoto imagwirizana mwachindunji ndi kuchuluka kwa molekyulu yofunidwa, zomwe zimathandiza kuti pakhale kusanthula kwa kuchuluka.

Kuyesa kwa ntchito ya enzyme: 3,3', 5,5'-Tetramethylbenzidine sulfate ingagwiritsidwe ntchito poyesa ntchito ya enzyme. Mwa kuyika chitsanzo cha enzyme ndi substrate, momwe enzyme imagwirira ntchito zimatha kuyang'aniridwa ndi kusintha kwa mtundu. Ntchito yayikulu ya enzyme imabweretsa mtundu wabuluu wamphamvu, zomwe zimathandiza kudziwa momwe enzyme imagwirira ntchito komanso maphunziro oletsa.

Immunohistochemistry: Ingagwiritsidwe ntchito popaka utoto mu immunohistochemistry (IHC). Imagwiritsidwa ntchito ngati chromogen ya ma antibodies oyambira kapena achiwiri olembedwa ndi horseradish peroxidase, zomwe zimapangitsa kuti pakhale malo owoneka bwino abuluu pamalo pomwe antigen-antibody imagwirira ntchito.

Kafukufuku wa biochemical: 3,3', 5,5'-Tetramethylbenzidine sulfate imagwiritsa ntchito kafukufuku wosiyanasiyana wa biochemical, makamaka pakupeza ndi kuyeza ntchito za ma enzyme, kuyanjana kwa mapuloteni, komanso kuyanjana kwa mapuloteni ndi DNA.