4-Amino-3-hydrazino-1,2,4-triazol-5-thiol CAS:28836-03-5

Zotsatira za 3,3'-Diaminobenzidine (DAB) ndikupanga mtundu wa bulauni ikakumana ndi ma enzyme a peroxidase. Kusintha kwa mtundu kumeneku kumalola kuwonetsa ma antigen kapena ma biomolecule omwe ali ndi chidwi ndi immunohistochemistry (IHC) ndi immunocytochemistry (ICC) staining assays.DAB nthawi zambiri imagwiritsidwa ntchito mu kafukufuku wa zamankhwala ndi ntchito zowunikira kuti azindikire ndikupeza ma antigen kapena mapuloteni enaake mkati mwa zitsanzo zamoyo. Mwa kuwonjezera DAB ngati substrate ndi reaction yotsatira ndi ma enzyme a peroxidase, brown precipitate imapangidwa pamalo omwe ma antigen amafunidwa. Kugwiritsa ntchito DAB staining ndikothandiza kwambiri pophunzira matenda ndi mikhalidwe yosiyanasiyana. Kumathandiza ofufuza ndi akatswiri a matenda kuwona ndi kusanthula kufalikira, kuchuluka, ndi malo a ma biomolecule enaake m'maselo kapena minofu. Chidziwitsochi chimathandiza kumvetsetsa njira zochizira matenda, kuphunzira njira zamaselo, ndi kuzindikira zomwe zingatheke pozindikira matenda kapena kuchiza. Kuphatikiza apo, utoto wa DAB ungagwiritsidwe ntchito m'njira zosiyanasiyana zamamolekyulu kuti afufuze momwe mapuloteni ndi mapuloteni amagwirira ntchito, kuzindikira momwe mapuloteni amagwirira ntchito, kudziwa kupezeka kwa zizindikiro zinazake, ndikuwunika momwe njira zochiritsira zimagwirira ntchito. Chifukwa cha utoto wake wodalirika komanso wolimba, komanso kugwirizana kwake ndi njira zosiyanasiyana zodziwira matenda ndi njira zama microscopy, DAB yakhala gawo logwiritsidwa ntchito kwambiri la chromogenic mu immunohistochemistry ndi immunocytochemistry assays.