4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

Enzyme Substrate: pNAG imagwiritsidwa ntchito kwambiri ngati gawo laling'ono la michere yosiyanasiyana yomwe imakhudzidwa ndi hydrolysis ya β-D-glucoside bond.Ma enzymes akamadula molekyulu ya pNAG, imatulutsa p-nitrophenol.Izi zimathandiza ofufuza kuyeza ndi kuwerengera ntchito ya enzymatic.

Ma enzyme Activity Assays: Hydrolysis ya pNAG ndi ma enzymes ena amatha kuzindikirika ndikuyezedwa spectrophotometrically.Izi zimapangitsa pNAG kukhala yoyenera kuyeserera kwa ma enzyme, komwe kuchuluka kwa p-nitrophenol komwe kumapangidwa kumayenderana mwachindunji ndi ntchito ya enzymatic.

Kuyang'ana Kwambiri: pNAG imagwiritsidwa ntchito poyesa mayeso apamwamba kwambiri kuti azindikire ndikuwonetsa ma enzyme inhibitors kapena activator.Powunika momwe ma enzyme osiyanasiyana amakhudzira ntchito ya enzymatic, ofufuza amatha kuzindikira omwe angakhale okonda mankhwala osokoneza bongo kapena ma modulators a enzyme.

Gene Expression Studies: pNAG imagwiritsidwanso ntchito mu kafukufuku wa biology ya maselo kuti aphunzire za jini ndi malamulo.Poyesa ntchito ya enzymatic ya michere inayake yogwiritsa ntchito pNAG ngati gawo lapansi, ofufuza amatha kufufuza momwe ma jini amagwirira ntchito ndi ntchito.