Nitrotetrazolium Blue Chloride CAS:298-83-9

Nitrotetrazolium Blue Chloride (NBT) ndi chizindikiro cha redox chomwe chimagwiritsidwa ntchito kwambiri poyesa zamoyo ndi zamankhwala. Ndi ufa wachikasu wopepuka womwe umasanduka buluu ukachepetsedwa, zomwe zimapangitsa kuti ukhale wothandiza pozindikira kukhalapo kwa ma enzyme ena ndi kagayidwe kachakudya.

Chotsatira chachikulu cha NBT ndi kupangika kwa buluu la formazan precipitate pamene limachepetsedwa ndi ma enzyme ena. Kusintha kwa mtundu kumeneku kumalola kuzindikira zochitika za enzyme m'maso kapena m'ma spectrophotometric.

NBT ili ndi ntchito zosiyanasiyana pofufuza ndi kuzindikira matenda. Nazi zina mwa ntchito zake zazikulu:

Kuyesa kwa ntchito ya enzyme: NBT ingagwiritsidwe ntchito poyesa ntchito ya dehydrogenases, zomwe zimakhudzidwa ndi njira monga kupuma kwa maselo ndi kagayidwe kachakudya. Mwa kuyang'anira kuchepa kwa NBT kukhala formazan, ofufuza amatha kuwunika ntchito ya ma enzyme awa.

Kuwunika momwe maselo a chitetezo chamthupi amagwirira ntchito: NBT imagwiritsidwa ntchito kwambiri mu mayeso ochepetsa NBT kuti aone momwe maselo a chitetezo chamthupi amapumira, makamaka ma phagocyte. Kuwunikaku kumayesa kuthekera kwa maselowa kupanga mitundu ya okosijeni yogwira ntchito, yomwe ingachepetse NBT ndikupanga buluu.

Kafukufuku wa microbiology: NBT imagwiritsidwa ntchito mu microbiology pophunzira kagayidwe ka tizilombo toyambitsa matenda ndikuwunika momwe ma enzyme enaake amagwirira ntchito. Mwachitsanzo, yagwiritsidwa ntchito pozindikira mabakiteriya otchedwa bacterial nitrate reductases kapena mabakiteriya opanga formazan.

Maphunziro okhudza moyo wa maselo: Kuchepetsa NBT kumathandiza ofufuza kuwunika momwe maselo amagwirira ntchito komanso momwe maselo amagwirira ntchito. Poyesa kuchuluka kwa mphamvu ya chinthu cha blue formazan, n'zotheka kudziwa kuchuluka kwa maselo omwe ali ndi moyo mu chitsanzo chomwe chaperekedwa.