4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Zotsatira: ONPG ndi gawo logwiritsidwa ntchito makamaka kuzindikira kukhalapo ndi ntchito ya enzyme ya β-galactosidase. Pamene enzyme ya β-galactosidase ilipo ndipo ikugwira ntchito, imagawa ONPG kukhala zinthu ziwiri: o-nitrophenol ndi galactose derivative. Kutulutsidwa kwa o-nitrophenol kumabweretsa kusintha kwa mtundu wachikasu, komwe kumatha kuyezedwa pogwiritsa ntchito spectrophotometer.

Kugwiritsa Ntchito: ONPG ili ndi ntchito zingapo mu kafukufuku wa sayansi ya maselo ndi sayansi ya zamoyo:

Kudziwa ntchito ya β-galactosidase: ONPG imagwiritsidwa ntchito kwambiri poyesa ndikuwerengera ntchito ya enzyme ya β-galactosidase. Kuchuluka kwa kapangidwe ka o-nitrophenol, komwe kumagwirizana mwachindunji ndi ntchito ya enzyme, kumatha kuyezedwa pogwiritsa ntchito spectrophotometric.

Kufotokozera ndi kulamulira majini: ONPG nthawi zambiri imagwiritsidwa ntchito poyesa zokhudzana ndi kufotokozera majini ndi maphunziro okhudza kulamulira. Imagwiritsidwa ntchito nthawi zambiri poyesa mapuloteni osakanikirana, monga njira yolumikizira ya lacZ yomwe imagwiritsidwa ntchito kwambiri, kuti iphunzire kufotokozera majini motsogozedwa ndi othandizira enaake. Ntchito ya beta-galactosidase yoyesedwa pogwiritsa ntchito ONPG imapereka chidziwitso cha kuchuluka kwa kufotokozera majini.

Kuyesa ntchito ya β-galactosidase: ONPG ingagwiritsidwe ntchito ngati njira yowunikira ya colorimetric mu ukadaulo wa DNA wophatikizana kuti izindikire kukhalapo ndi magwiridwe antchito a jini la LacZ, lomwe limalemba β-galactosidase. Njira yowunikirayi imathandiza kuzindikira ma clones omwe ali ndi jini lofunikira.

Maphunziro a kinetics a enzyme: ONPG imathandizanso pophunzira kinetics ya enzyme ya β-galactosidase. Poyesa kuchuluka kwa reaction ya enzyme-substrate pamlingo wosiyanasiyana wa substrate, n'zotheka kudziwa magawo a kinetic monga Michaelis-Menten constants (Km) ndi maximum reaction rates (Vmax).