4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Kuyesa kwa Enzyme Activity: Kuchuluka kwa hydrolysis ya 4-Nitrophenyl-beta-D-glucopyranoside ndi ma enzyme monga β-glucosidase kungagwiritsidwe ntchito poyesa ntchito ya enzyme. Kutulutsidwa kwa 4-nitrophenol kumatha kuwerengedwa pogwiritsa ntchito spectrophotometry, zomwe zimathandiza kudziwa momwe enzyme kinetics imagwirira ntchito komanso maphunziro oletsa.

Kuyezetsa kwa majini ndi ma reporter: 4-Nitrophenyl-beta-D-glucopyranoside ikhoza kugwirizanitsidwa ndi ma molecular tag enaake omwe amaphatikizidwa mu fusion proteins. Ntchito ya enzyme ya fusion protein imatha kuwerengedwa mosavuta poyesa kutulutsidwa kwa 4-nitrophenol, kupereka chidziwitso pa gene expression ndi protein interactions.

Kupeza mankhwala ndi kuyezetsa kuchuluka kwa mankhwala: Kuyezetsa kwa 4-Nitrophenyl-beta-D-glucopyranoside kungagwiritsidwe ntchito ngati njira yodziwira matenda ena. Izi zimathandiza kuyezetsa mwachangu m'malaibulale akuluakulu kuti adziwe zinthu zomwe zingalepheretse ma enzyme kapena zinthu zina.

Kuyezetsa matenda: Matenda ena amagwirizanitsidwa ndi kusintha kwa ntchito zina za enzyme. Pogwiritsa ntchito hydrolysis ya 4-Nitrophenyl-beta-D-glucopyranoside ndi ma enzyme enaake ngati mayeso oyezetsa matenda, matendawa amatha kupezeka kapena kuyang'aniridwa. Izi zikuphatikizapo matenda okhudzana ndi matenda osungira lysosomal ndi matenda a chiwindi.