5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt CAS:18656-96-7

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide Mchere wa cyclohexylammonium, womwe umadziwikanso kuti X-glucuronide, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri mu ma molecular biology ndi ma enzymology. Ndi gawo la chromogenic lomwe nthawi zambiri limagwiritsidwa ntchito kuzindikira ntchito ya enzyme beta-D-glucuronidase.

Phala ili limapangidwa ndi gulu la indole lolumikizidwa ku gawo la glucuronic acid kudzera mu glycosidic bond. Bromo ndi chloro substituents zimapereka kukhazikika ndikuwonjezera kusungunuka kwa phala.

Mukayikidwa mu chitsanzo chokhala ndi beta-D-glucuronidase, enzyme imadula glycosidic bond, ndikutulutsa indole moiety. Izi zimapangitsa kuti pakhale kusintha kwa buluu kapena mtundu, zomwe zimapangitsa kuti ntchito ya enzyme izioneka mosavuta kapena spectrophotometric izindikire mosavuta.

Chifukwa cha kudziwika kwake kwa beta-D-glucuronidase, X-glucuronide imagwiritsidwa ntchito kwambiri mu njira za biology ya mamolekyu monga kuyesa kusintha kwa bakiteriya ndi yisiti, kuyesa majini a reporter, ndi kuyika utoto wa histochemical. Imapereka njira yosavuta komanso yodziwira bwino kupezeka kapena kuwonetsedwa kwa beta-D-glucuronidase ndipo nthawi zambiri imagwiritsidwa ntchito limodzi ndi mayeso ena a majini kapena a biochemical.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Zotsatira za mchere wa 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium (X-glucuronide) ndi kuthekera kwake kupanga kusintha kwa buluu kapena mtundu pamaso pa enzyme beta-D-glucuronidase. Kachitidwe ka enzyme aka kamachitika pamene mgwirizano wa glycosidic pakati pa gulu la indole ndi gawo la glucuronic acid umasweka ndi beta-D-glucuronidase.

Kugwiritsa ntchito X-glucuronide kumachitika makamaka mu kafukufuku wa zamoyo zama molekyulu ndi ma enzymology. Nthawi zambiri amagwiritsidwa ntchito ngati gawo lothandizira kuzindikira momwe beta-D-glucuronidase imagwirira ntchito. Ntchito zina zapadera ndi izi:

Kuyesa majini a mtolankhani: X-glucuronide nthawi zambiri imagwiritsidwa ntchito ngati gawo la kafukufuku wa majini a mtolankhani komwe beta-D-glucuronidase imagwiritsidwa ntchito ngati enzyme yolemba nkhani. Mwa kuphatikiza dera lolimbikitsa kapena kuyika ma code a jini yosangalatsa ku jini ya beta-D-glucuronidase, ofufuza amatha kuyeza momwe jiniyo imagwirira ntchito poyang'anira kupangika kwa buluu kapena kusintha kwa mtundu pamene X-glucuronide yowonjezeredwa.

Kuyesa kusintha kwa bakiteriya ndi yisiti: X-glucuronide ingagwiritsidwe ntchito kuzindikira kusintha kwabwino kwa mabakiteriya kapena yisiti pogwiritsa ntchito vector yokhala ndi jini ya beta-D-glucuronidase. Maselo osinthika omwe akuwonetsa enzyme amatha kupha X-glucuronide ndi madzi, zomwe zimapangitsa kuti mtundu usinthe kapena kuoneka bwino.

Kupaka utoto wa histochemical: X-glucuronide imagwiritsidwa ntchito mu njira zopaka utoto wa histochemical kuti iwonetse kukhalapo ndi kufalikira kwa ntchito ya beta-D-glucuronidase m'maselo kapena minofu. Kupaka utoto kumeneku kumathandiza kuti maselo omwe akuwonetsa enzyme apezeke komanso kuti adziwe komwe kuli.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C26H36BrClN2O7
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	18656-96-7
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.