5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ndi mankhwala omwe amagwiritsidwa ntchito m'maphunziro osiyanasiyana a biochemical, makamaka pozindikira ndikuwonetsa momwe ma enzyme amagwirira ntchito. Ndi gawo lomwe limatha kusungunuka ndi ma enzyme enaake, zomwe zimapangitsa kuti pakhale kutulutsa mankhwala obiriwira kapena owala.

Kapangidwe kameneka kamagwiritsidwa ntchito kwambiri poyesa kuti azindikire kukhalapo ndi ntchito ya ma enzyme monga beta-galactosidase ndi beta-glucuronidase. Ma enzyme amenewa amagawa magulu a acetyl ndi glucosaminide kuchokera ku substrate, zomwe zimapangitsa kuti pakhale chromophore yabuluu kapena yobiriwira.

Kapangidwe kapadera ka 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide kamalola kuzindikira mosavuta ndi kuwerengera ntchito ya enzyme. Kugwiritsa ntchito kwake m'njira zosiyanasiyana zoyesera, kuphatikizapo histochemistry, immunohistochemistry, ndi ma cell-based assays, kwathandiza kumvetsetsa bwino ntchito za enzyme.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) ndi gawo la chromogenic lomwe limagwiritsidwa ntchito kwambiri pozindikira ntchito ya beta-glucuronidase (GUS). GUS ndi enzyme yomwe imapezeka m'zamoyo zosiyanasiyana, kuphatikizapo mabakiteriya, zomera, ndi nyama zoyamwitsa. X-Gluc imagwiritsidwa ntchito nthawi zambiri mu mayeso a GUS reporter ndi kafukufuku wa biology ya mamolekyulu.

Kugwiritsa ntchito kwakukulu kwa X-Gluc ndi njira zopaka utoto wa histochemical, komwe kumatha kuwona momwe enzyme ya GUS imagwirira ntchito komanso momwe imagwirira ntchito. Gawoli limatha kulowa m'maselo ndipo limasungunuka ndi GUS, zomwe zimapangitsa kuti pakhale mtundu wa buluu kapena chinthu chosasungunuka. Kupaka utoto wabuluu kumeneku kumathandiza ofufuza kuzindikira ndikupeza momwe GUS imagwirira ntchito m'maselo, minofu, ndi zamoyo zonse.

X-Gluc ingagwiritsidwenso ntchito poyesa kuchuluka kwa zinthu kuti iyese ntchito ya enzyme ya GUS. Mphamvu ya mtundu wabuluu kapena kuchuluka kwa chinthu chomwe chapangidwa kungagwirizane ndi kuchuluka kwa momwe GUS imagwirira ntchito kapena ntchito yake ya enzyme.

Kuphatikiza apo, X-Gluc yagwiritsidwa ntchito pofufuza za majini a zomera kuti iphunzire momwe majini amagwirira ntchito, momwe amalimbikitsira, komanso kusintha kwa zomera. Yagwiritsidwanso ntchito m'machitidwe a mabakiteriya popanga ndi kuzindikira mapuloteni osakanikirana a GUS.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C16H18BrClN2O6
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	4264-82-8
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.