5,5-Dithiobis (2-nitrobenzoic acid) CAS:69-78-3

5,5-Dithiobis (2-nitrobenzoic acid), yomwe imadziwikanso kuti DTNB kapena Ellman's reagent, ndi mankhwala omwe amagwiritsidwa ntchito kwambiri pofufuza zamankhwala ndi zamankhwala. Ndi ufa wachikasu, wa kristalo womwe umasungunuka kwambiri m'madzi ndi zinthu zachilengedwe.

DTNB imagwiritsidwa ntchito makamaka poyesa kuchuluka kwa magulu a sulfhydryl (-SH) m'mapuloteni ndi ma peptide. Chosakanizacho chimagwira ntchito ndi ma thiol, zomwe zimapangitsa kuti pakhale disulfide yosakanikirana ndikutulutsa 2-nitro-5-thiobenzoate, yomwe ili ndi mtundu wachikasu womwe ungayesedwe ndi spectrophotometric. Poyesa kuchuluka kwa mtundu wachikasu, n'zotheka kuyerekeza kuchuluka kwa ma thiols mu chitsanzo. Njirayi nthawi zambiri imagwiritsidwa ntchito pophunzira momwe mapuloteni amagwirira ntchito, kupindika kwa mapuloteni, ndi machitidwe a enzymatic omwe amakhudza magulu a thiol.

Kuwonjezera pa kuwerengera kwa thiol, DTNB yagwiritsidwa ntchito m'magwiritsidwe ena, monga kuwunika momwe maselo alili, kuphunzira kukhazikika kwa mankhwala achitsulo-sulfure, komanso kuyeza ntchito ya ma enzyme osiyanasiyana.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kuyeza kwa Thiol: DTNB nthawi zambiri imagwiritsidwa ntchito poyesa kuchuluka kwa ma thiol m'ma protein samples. Izi ndizofunikira pophunzira momwe mapuloteni amagwirira ntchito, kupindika kwa mapuloteni, ndi momwe ma enzyme amagwirira ntchito m'magulu a thiol. Kuyankha kwa colorimetric kwa DTNB kumalola kuyeza kwa ma thiol kudzera mu muyeso wa spectrophotometric.

Kuwunika kwa Redox State: DTNB yagwiritsidwa ntchito powunika momwe redox ilili mkati mwa maselo, minofu, kapena zitsanzo zamoyo. Poyesa kusintha kwa kuchuluka kwa thiol, ofufuza amatha kupeza chidziwitso cha kupsinjika kwa okosijeni, ntchito ya antioxidant, ndi njira zolumikizirana za redox.

Kuyesa kwa Enzyme: Kuchita bwino kwa DTNB ndi thiols kumapangitsa kuti ikhale chida chofunikira kwambiri poyesa ma enzyme. Ma enzyme ambiri, monga glutathione-S-transferases, cystathionine-γ-lyase, ndi thioredoxin reductase, amadalira thiol ndipo amatha kuwerengedwa kapena kuyesedwa pogwiritsa ntchito DTNB.

Kusanthula Kukhazikika kwa Ma Metal-Sulfur Compounds: DTNB imagwiritsidwa ntchito poyesa kukhazikika ndi kuyanjana kwa ma metal-sulfur complexes ndi ma metalloproteins. Ma metalloproteins amatha kuzindikira ndikuwerengera kupezeka kwa ma thiols omwe amakhudzidwa ndi chelation ya ma ayoni achitsulo, kupereka chidziwitso chofunikira chokhudza kuyanjana kwa metal-sulfur ndi chemistry yolumikizana.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C14H8N2O8S2
Kuyesa	99%
Maonekedwe	Cholimba chachikasu
Nambala ya CAS	69-78-3
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.