BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

Mchere wa 5-Bromo-4-chloro-3-indolyl phosphate disodium ndi mankhwala opangidwa omwe amagwiritsidwa ntchito kwambiri mu kafukufuku wa biochemical ngati gawo la chromogenic kuti azindikire ntchito ya ma enzymes a alkaline phosphatase. Nthawi zambiri amatchedwa BCIP mwachidule.

Pamene alkaline phosphatase ikugwira ntchito pa BCIP, imadula gulu la phosphate, zomwe zimapangitsa kuti pakhale mtundu wa buluu-wofiirira. Kusintha kwa mtundu kumeneku kumathandiza ofufuza kuona ndi kuyeza kupezeka kwa alkaline phosphatase mu chitsanzo.

BCIP imagwiritsidwa ntchito nthawi zambiri m'njira zosiyanasiyana, kuphatikizapo immunohistochemistry, njira zofufuzira mapuloteni ndi nucleic acid, ndi mayeso a immunosorbent omwe amalumikizidwa ndi enzyme (ELISAs)..

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Zotsatira:

BCIP ndi chinthu chopanda mtundu chomwe chimasanduka mtundu wa buluu-wofiirira pambuyo pa ntchito ya alkaline phosphatase. Kusintha kwa mtundu kumeneku kumalola kuzindikira ndi kuwerengera ntchito ya enzyme.

Mapulogalamu:

Kuyesa kwa Enzyme: BCIP imagwiritsidwa ntchito mu mayeso osiyanasiyana a biochemical kuti muyese ntchito ya alkaline phosphatase. Izi zikuphatikizapo kuzindikira ntchito ya phosphatase mu maselo a lysates, ma enzyme oyeretsedwa, kapena mayeso a in vitro.

Immunohistochemistry: BCIP ingagwiritsidwe ntchito kuwona ma antibodies okhala ndi chizindikiro cha alkaline phosphatase m'magawo a minofu kapena pamwamba pa maselo. Njira imeneyi nthawi zambiri imagwiritsidwa ntchito pokonza ma immunostaining protocols.

Njira zochotsera mabala: BCIP imagwiritsidwa ntchito mu Western blotting, Southern blotting, ndi Northern blotting kuti ipeze mapuloteni enaake kapena nucleic acids omwe alembedwa ndi ma probe a alkaline phosphatase-conjugated.

Kusakaniza kwa malo: BCIP ingagwiritsidwe ntchito kuwonetsa mawonekedwe a majini m'maselo kapena minofu kudzera mu njira zosakaniza za malo.

Kuyesa matenda: BCIP imagwiritsidwa ntchito ngati gawo la zida zosiyanasiyana zodziwira matenda kuti azindikire alkaline phosphatase. Zida zimenezi zimagwiritsidwa ntchito m'ma laboratories azachipatala kuti azindikire matenda ndikuwunika.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C8H7BrClNNaO4P
Kuyesa	99%
Maonekedwe	Ufa wachikasu wotumbululuka
Nambala ya CAS	102185-33-1
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.