BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine mchere CAS:6578-06-9

Mchere wa 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine ndi mankhwala omwe amagwiritsidwa ntchito kwambiri mu kafukufuku wa zamoyo zama molekyulu ndi biochemistry. Nthawi zambiri amagwiritsidwa ntchito mu enzyme-linked immunosorbent assays (ELISA) ndi njira zolembera mapuloteni.

Kapangidwe kameneka ndi gawo la alkaline phosphatase, enzyme yomwe imagwiritsidwa ntchito kwambiri mu biology ya mamolekyulu pozindikira kukhalapo kwa mamolekyulu kapena mapuloteni enaake kudzera mu colorimetric kapena chemiluminescent reactions.

Pamene enzyme ya alkaline phosphatase ilipo, imadula magulu a phosphate kuchokera ku mchere wa 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine, zomwe zimapangitsa kuti utoto wabuluu kapena wofiirira upangidwe. Kusintha kwa mtundu kumeneku kumatha kuzindikirika mosavuta ndikuwerengedwa, zomwe zimathandiza kuyeza ntchito ya enzyme kapena kukhalapo kwa mamolekyu omwe akufuna.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kuchotsa magazi m'thupi: BCIP imagwiritsidwa ntchito kuzindikira ndikuwona mapuloteni omwe amasamutsidwa kupita ku nembanemba pambuyo pa gel electrophoresis. Ma antibodies achiwiri a Alkaline phosphatase-conjugated amagwiritsidwa ntchito kumangirira ku ma antibodies oyambira, ndipo pambuyo pake kulowetsedwa ndi BCIP kumapangitsa kuti pakhale blue precipitate pamalo omwe mapuloteni ali.

Immunohistochemistry: BCIP imagwiritsidwa ntchito mu njira zoyezera utoto wa immunohistochemical kuti izindikire kupezeka kwa mapuloteni enaake m'minofu. Ma antibodies achiwiri a Alkaline phosphatase-conjugated amagwiritsidwa ntchito kumangirira ku ma antibodies oyambira, ndipo chithandizo cha BCIP chimapanga mtundu wabuluu pamaso pa enzyme, kusonyeza malo a mapuloteni mkati mwa chitsanzo cha minofu.

Kusakaniza kwa malo: BCIP imagwiritsidwanso ntchito pozindikira ma nucleic acid sequences m'maselo kapena minofu yokhazikika. Ma probe osakaniza amalembedwa ndi alkaline phosphatase, ndipo powonjezera BCIP, kupezeka kwa mRNA kapena DNA yomwe ikufunidwa kumawonetsedwa ndi mtundu wabuluu.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C8H6BrClNO4P.C7H9N
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	6578-06-9
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.