Concanavalin A CAS:11028-71-0 Mtengo wa Wopanga

Concanavalin A (Con A) ndi lectin, yomwe ndi mtundu wa puloteni yomwe imatha kumangirira makamaka ku mamolekyu ena a shuga. Imachokera ku mbewu za chomera cha jackbean (Canavalia ensiformis).

Con A imagwirizana kwambiri ndi zotsalira za shuga za mannose ndi glucose ndipo imatha kumangirira ku ma glycoprotein ndi ma glycolipids okhala ndi shuga awa. Imapanga ma complexes osinthika ndi mamolekyu awa kudzera mu mgwirizano wa carbohydrate ndi mapuloteni.

Chifukwa cha kuthekera kwake kogwirizanitsa shuga, Con A imagwiritsidwa ntchito kwambiri mu kafukufuku wa zamoyo ndi matenda. Yagwiritsidwa ntchito kwambiri ngati chida chophunzirira ma glycoprotein, shuga pamwamba pa maselo, ndi kuyanjana kwa chakudya chochokera ku chakudya. Ingagwiritsidwe ntchito kupatula ndi kuyeretsa ma glycoprotein pogwiritsa ntchito affinity chromatography, komanso kuzindikira ndikuwerengera magawo enaake a shuga pamwamba pa maselo kapena m'zosakaniza zovuta.

Con A ilinso ndi mphamvu zowongolera chitetezo chamthupi. Ikhoza kuyambitsa ndi kuchulukitsa kwa maselo ena a chitetezo chamthupi, monga ma T lymphocytes, ndipo yaphunziridwa kuti ingagwiritsidwe ntchito pochiza matenda a chitetezo chamthupi komanso njira zodzitetezera.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kulumikizana kwa Maselo: Chimodzi mwa zotsatira zodziwika bwino za Con A ndi kuthekera kwake kuyambitsa kusonkhana kwa maselo. Izi zakhala zothandiza pophunzira kukhudzana kwa maselo ndi momwe maselo amagwirira ntchito pamwamba pa maselo.

Kupatula Glycoprotein: Con A imagwirizana makamaka ndi ma glycoprotein omwe ali ndi terminal mannose ndi zotsalira za shuga. Kapangidwe kameneka kagwiritsidwa ntchito popatula ndikuyeretsa ma glycoprotein kuchokera ku zosakaniza zovuta, monga madzi achilengedwe kapena ma lysates a maselo, pogwiritsa ntchito Con A affinity chromatography.

Kuzindikira Ma Glycoproteins: Con A ingagwiritsidwe ntchito kuzindikira ndi kuwerengera ma glycoproteins m'zitsanzo. Pogwiritsa ntchito Con A yolumikizidwa ndi ma fluorescent kapena enzymatic tag, n'zotheka kuwona kapena kuyeza kuchuluka kwa ma glycoproteins omwe ali mu chitsanzo kudzera mu fluorescence microscopy kapena enzyme-linked immunosorbent assay (ELISA) njira, motsatana.

Kulemba Malembo Pamwamba pa Maselo: Chifukwa cha kugwirizana kwake ndi shuga pamwamba pa maselo, Con A ingagwiritsidwe ntchito kulemba mitundu inayake ya maselo kapena kuphunzira machitidwe a glycosylation pamwamba pa maselo. Con A yolumikizidwa ndi fluorophores kapena ma tag ena imalola kuwona ndi kusanthula maselo kudzera mu njira monga flow cytometry kapena fluorescence microscopy.

Kusinthasintha kwa Chitetezo cha Mthupi: Con A ili ndi mphamvu zowongolera chitetezo chamthupi ndipo ingakhudze ntchito ya maselo a chitetezo chamthupi. Ikhoza kuyambitsa kuyambika ndi kufalikira kwa ma T lymphocytes, zomwe zimapangitsa kuti ma cytokines ndi mayankho ena a chitetezo chamthupi apangidwe. Mphamvu imeneyi yafufuzidwa mu chithandizo cha chitetezo chamthupi ndi kupanga katemera, komwe Con A yagwiritsidwa ntchito kukulitsa mayankho a chitetezo chamthupi kapena kusintha kulekerera kwa chitetezo chamthupi.

Chida Chofufuzira: Con A ndi chida chogwiritsidwa ntchito kwambiri m'magawo osiyanasiyana ofufuza zamoyo. Chagwiritsidwa ntchito pophunzira momwe chakudya chimagwirira ntchito ndi mapuloteni, kumamatira kwa maselo, njira za glycosylation, ndi mayankho a chitetezo chamthupi. Kutha kwake kumalumikizana ndi shuga winawake kumapangitsa kuti chikhale chofunikira pophunzira ndikusintha ma glycoprotein ndi ma glycolipids.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C15H24
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	11028-71-0
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.