DAPI (4,6-Diamidini-2-phenylindole dihydrochloride) CAS:28718-90-3

DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) ndi utoto wa fluorescent womwe umagwiritsidwa ntchito kwambiri pofufuza zamoyo kuti uwonetse DNA. Umalumikizana ndi mzere waung'ono wa DNA yokhala ndi zingwe ziwiri ndipo umatulutsa kuwala kwabuluu ukaunikiridwa ndi kuwala kwa ultraviolet. DAPI nthawi zambiri imagwiritsidwa ntchito mu microscopy ya fluorescence kuti ipange utoto wa maselo, zomwe zimapangitsa kuti ikhale chida chofunikira pophunzira kapangidwe ndi kapangidwe ka DNA mkati mwa maselo. Imagwiritsidwanso ntchito mu flow cytometry kuti ifufuze kuchuluka kwa DNA m'maselo osiyanasiyana komanso njira zina zowonetsera ndi kuwerengera DNA. Kugwirizana kwakukulu kwa DAPI ndi DNA ndi mawonekedwe ake enieni a fluorescent kumapangitsa kuti ikhale chida chogwiritsidwa ntchito kwambiri komanso chogwiritsidwa ntchito kwambiri mu kafukufuku wa mamolekyulu ndi maselo.