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Zogulitsa

DDT CAS: 3483-12-3 Mtengo Wopanga

DL-Dithiothreitol, yomwe imadziwikanso kuti DTT, ndi chochepetsera chomwe chimagwiritsidwa ntchito pofufuza za biochemical ndi ma cell biology.Ndi kamolekyu yaing'ono yokhala ndi gulu la thiol (lokhala ndi sulfure) kumapeto kulikonse.

DTT imagwiritsidwa ntchito mobwerezabwereza kuswa zomangira za disulfide m'mapuloteni, zomwe zimathandizira kuwulutsa kapena kuzimitsa.Kuchepetsa kwa zomangira za disulfide ndikofunikira pamachitidwe osiyanasiyana a labotale monga kuyeretsa mapuloteni, gel electrophoresis, ndi maphunziro a protein.DTT itha kugwiritsidwanso ntchito kuteteza magulu a thiol ndikuletsa oxidation panthawi yoyesera.

DTT nthawi zambiri imawonjezeredwa ku mayankho oyeserera pang'onopang'ono, ndipo ntchito yake imadalira kukhalapo kwa mpweya.Ndikofunika kusamalira DTT mosamala chifukwa imakhudzidwa ndi mpweya, kutentha, ndi chinyezi, zomwe zingachepetse mphamvu zake.


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Zogulitsa Tags

Kugwiritsa Ntchito ndi Zotsatira

Kuchepetsa kwa Disulfide Bond: DTT imagwiritsidwa ntchito makamaka kuswa ma bond a disulfide, omwe ndi ma covalent bond omwe amapangidwa pakati pa zotsalira ziwiri za cysteine ​​​​mu mapuloteni.Pochepetsa zomangira izi, DTT imathandizira mapuloteni a denature, kuthandizira kuphunzira kapangidwe kawo ndi ntchito zawo.

Kupinda kwa Mapuloteni: DTT ikhoza kuthandizira pakumanga bwino kwa mapuloteni poletsa mapangidwe olakwika a disulfide.Imachepetsa zomangira zamtundu uliwonse zomwe sizili zamtundu wa disulfide zomwe zimatha kupanga pakupindika kwa mapuloteni, zomwe zimapangitsa kuti puloteniyo itenge mawonekedwe ake.

Ntchito ya Enzyme: DTT imatha kuyambitsa ma enzymes ena pochepetsa ma inhibitory disulfide bond omwe alipo.Kuphatikiza apo, DTT imatha kuletsa makutidwe ndi okosijeni a zotsalira za cysteine, zomwe zingakhale zofunikira pakuchita kwa ma enzyme.

Kupanga Ma Antibody: DTT imawonjezeredwa nthawi zambiri kuti muchepetse zomangira za disulfide panthawi yopanga ma antibodies.Zimathandiza kupewa mapangidwe olakwika a disulfide zomangira, zomwe zingalepheretse kumanga koyenera kwa antigen.

Mapuloteni Okhazikika: DTT ikhoza kugwiritsidwa ntchito kulimbitsa mapuloteni poletsa okosijeni kapena kuphatikizika kwawo.Zimathandizira kusunga kuchepa kwa mapuloteni panthawi yosungirako ndi kuyesa njira.

Kuchepetsa Ma Agents mu Molecular Biology: DTT imagwiritsidwa ntchito nthawi zambiri munjira zosiyanasiyana zamamolekyulu monga kutsatizana kwa DNA, PCR, ndi kuyeretsa mapuloteni.Zingathandize kusunga mkhalidwe wochepetsedwa wa zigawo zofunika kwambiri, kuonetsetsa zotsatira zabwino zoyesera.

Product Chitsanzo

3483-12-3
3483-12-3-2

Kulongedza katundu:

6892-68-8-3

Zina Zowonjezera:

Kupanga C4H10O2S2
Kuyesa 99%
Maonekedwe White ufa
CAS No. 3483-12-3
Kulongedza Zing'onozing'ono ndi zambiri
Shelf Life zaka 2
Kusungirako Sungani pamalo ozizira ndi owuma
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