DDT CAS:3483-12-3 Mtengo wa Wopanga

DL-Dithiothreitol, yomwe imadziwikanso kuti DTT, ndi chinthu chochepetsa chomwe chimagwiritsidwa ntchito kwambiri mu kafukufuku wa zamoyo ndi za mamolekyulu. Ndi molekyulu yaying'ono yokhala ndi gulu la thiol (lokhala ndi sulfure) kumapeto kulikonse.

DTT imagwiritsidwa ntchito nthawi zambiri kuswa ma disulfide bonds m'mapuloteni, zomwe zimathandiza kuwatsegula kapena kuwachotsa. Kuchepetsa ma disulfide bonds kumeneku n'kofunika kwambiri m'njira zosiyanasiyana za labotale monga kuyeretsa mapuloteni, gel electrophoresis, ndi maphunziro a kapangidwe ka mapuloteni. DTT ingagwiritsidwenso ntchito kuteteza magulu a thiol ndikuletsa okosijeni panthawi yoyesera.

DTT nthawi zambiri imawonjezeredwa ku mayankho oyesera pang'ono, ndipo ntchito yake imadalira kupezeka kwa mpweya. Ndikofunikira kugwiritsa ntchito DTT mosamala chifukwa imakhudzidwa ndi mpweya, kutentha, ndi chinyezi, zomwe zingachepetse mphamvu yake.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kuchepetsa Ma Bond a Disulfide: DTT imagwiritsidwa ntchito makamaka kuswa ma bond a disulfide, omwe ndi ma bond ogwirizana omwe amapangidwa pakati pa ma residues awiri a cysteine m'mapuloteni. Mwa kuchepetsa ma bond awa, DTT imathandiza kusintha mapuloteni, zomwe zimathandiza kuphunzira kapangidwe kake ndi ntchito yake.

Kupinda Mapuloteni: DTT ingathandize kupindika bwino mapuloteni mwa kupewa kupanga kolakwika kwa disulfide bond. Imachepetsa ma bond aliwonse a disulfide omwe si achilengedwe omwe angapangidwe panthawi yopinda mapuloteni, zomwe zimathandiza kuti mapuloteniwo agwirizane ndi mawonekedwe ake enieni.

Ntchito ya Enzyme: DTT imatha kuyambitsa ma enzyme ena mwa kuchepetsa ma bond aliwonse oletsa disulfide omwe alipo. Kuphatikiza apo, DTT imatha kuletsa kukhuthala kwa ma residues ofunika a cysteine, omwe angafunike pa ntchito ya enzyme.

Kupanga Ma Antibody: DTT nthawi zambiri imawonjezeredwa kuti ichepetse ma disulfide bond panthawi yopanga ma antibodies. Zimathandiza kupewa mapangidwe a ma disulfide bond olakwika, zomwe zingalepheretse ma antigen kumangirira bwino.

Kukhazikitsa Mapuloteni: DTT ingagwiritsidwe ntchito kukhazikitsa mapuloteni mwa kupewa kusungunuka kapena kusakanizika. Imathandiza kusunga kuchepa kwa mapuloteni panthawi yosungira ndi kuyesa.

Zinthu Zochepetsa mu Molecular Biology: DTT nthawi zambiri imagwiritsidwa ntchito mu njira zosiyanasiyana za molecular biology monga DNA sequencing, PCR, ndi protein purification. Ingathandize kusunga kuchepa kwa zinthu zofunika kwambiri, ndikutsimikizira zotsatira zabwino kwambiri zoyeserera.

Chitsanzo cha Zamalonda

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C4H10O2S2
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	3483-12-3
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.