DNase I, kuchokera ku Bovine Pancreas CAS:9003-98-9

DNase I, yochokera ku kapamba wa ng'ombe, ndi enzyme yomwe imadula mamolekyu a DNA mwa kusakaniza ma phosphodiester bonds mu msana wa DNA. Chotsatira chachikulu cha DNase I ndikuwonongeka ndi kugawikana kwa DNA.

Kugwiritsa ntchito kwakukulu kwa DNase I kuli mu kafukufuku wa zamoyo zama molekyulu. Kawirikawiri imagwiritsidwa ntchito m'mayesero osiyanasiyana kuti ipatule ndikuyeretsa DNA, kuchotsa kuipitsidwa kwa DNA kuchokera ku zitsanzo za RNA, ndikusinthira DNA. DNase I imagwira ntchito bwino kwambiri pakuwononga DNA m'ma protein-DNA complexes, zomwe zimathandiza ofufuza kuphunzira momwe mapuloteni ndi DNA zimagwirira ntchito kapena kuchotsa mapuloteni ogwirizana ndi DNA.

Kuphatikiza apo, DNase I imagwiritsidwa ntchito nthawi zambiri poyesa DNA, zomwe zimathandiza kuzindikira madera a DNA omwe amamangiriridwa ndi mapuloteni enaake. Mwa kuchiza chitsanzo cha DNA ndi DNase I kenako ndikusanthula zidutswa za DNA zomwe zapezeka, ofufuza amatha kudziwa malo omangira mapuloteni omangira DNA.

DNase I imagwiritsidwanso ntchito pofufuza za chromatin, komwe imathandiza kugaya chromatin (DNA ndi mapuloteni ogwirizana nayo) kuti iphunzire kapangidwe kake ndi dongosolo lake. Mwa kugaya chromatin ndi DNase I, ofufuza amatha kuzindikira madera omwe ali osavuta kuwafikira komanso omwe amakhudzidwa ndi kugawanika kwa maselo, kupereka chidziwitso cha momwe majini amagwirira ntchito komanso kapangidwe ka chromatin.

Kuphatikiza apo, DNase I imagwiritsidwa ntchito pozindikira matenda, makamaka pozindikira ndikuwerengera DNA yopanda maselo m'magazi ozungulira. Pogwiritsa ntchito DNase I kugaya DNA yosazungulira, ndizotheka kusanthula ndikupeza njira zinazake za DNA zokhudzana ndi matenda kapena zolakwika za majini.