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N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid sodium mchere CAS:66992-27-6
N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid mchere wa sodium, womwe umadziwikanso kuti HEPES sodium salt, ndi mankhwala omwe amagwiritsidwa ntchito ngati pH buffering agent mu biological and chemical laboratories.Imathandizira kukhala ndi pH yokhazikika, ndikupangitsa kuti ikhale yoyenera kugwiritsa ntchito monga chikhalidwe cha ma cell, kuyesa kwa ma enzyme, maphunziro a protein, electrophoresis, ndi kupanga mankhwala.Mchere wa HEPES wa sodium umatsimikizira mikhalidwe yabwino kwambiri yazachilengedwe ndikuwonjezera kulondola komanso kudalirika kwa zotsatira zoyeserera.
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S-Butyrylthiocholine iodide CAS: 1866-16-6
S-Butyrylthiocholine iodide ndi mankhwala omwe amagwiritsidwa ntchito kwambiri poyesa biochemical ndi enzymatic.Ndi gawo lapansi la enzyme butyrylcholinesterase (BChE) ndipo amagwiritsidwa ntchito kuyeza ntchito yake.
Pamene S-Butyrylthiocholine iodide ndi hydrolyzed ndi BChE, imapanga thiocholine ndi butyric acid monga mankhwala.Kutulutsidwa kwa thiocholine kungayesedwe pogwiritsa ntchito spectrophotometric kapena fluorometric assay, kulola kuchuluka kwa ntchito za BChE.
S-Butyrylthiocholine iodide nthawi zambiri amagwiritsidwa ntchito m'machitidwe azachipatala ndi kafukufuku kuti ayese ntchito ya BChE mu zitsanzo monga plasma ya magazi kapena minofu.Itha kugwiritsidwa ntchito powunika momwe BChE imagwirira ntchito komanso zomwe zingachitike pazachilengedwe zosiyanasiyana, komanso kuzindikira ndi kuyang'anira matenda ena.
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ABTS (2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mchere wa diammonium) CAS:30931-67-0
Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate), yomwe nthawi zambiri imatchedwa ABTS, ndi gawo laling'ono la chromogenic lomwe limagwiritsidwa ntchito poyesa biochemical, makamaka pankhani ya enzymology.Ndiwopanga omwe amagwiritsidwa ntchito poyesa ntchito ya michere yosiyanasiyana, kuphatikiza peroxidase ndi oxidase.
ABTS imakhala yopanda mtundu m'mawonekedwe ake oxidized koma imasanduka yobiriwira yobiriwira ikaphatikizidwa ndi enzyme pamaso pa hydrogen peroxide kapena molekyulu ya oxygen.Kusintha kwamtundu kumeneku kumachitika chifukwa cha mapangidwe a cation, omwe amatenga kuwala mu mawonekedwe owoneka.
Zomwe zimachitika pakati pa ABTS ndi enzyme zimapanga chinthu chamitundu chomwe chitha kuyeza mowonera.Kuchuluka kwa mtunduwo kumagwirizana mwachindunji ndi ntchito ya enzymatic, kulola ofufuza kuti aunike mochulukirachulukira kwa enzyme kinetics, kuletsa kwa ma enzyme, kapena kuyanjana kwa enzyme-gawo.
ABTS ili ndi ntchito zingapo m'magawo osiyanasiyana, kuphatikiza zowunikira zamankhwala, kafukufuku wamankhwala, ndi sayansi yazakudya.Ndiwotcheru kwambiri ndipo imapereka mawonekedwe osiyanasiyana, kupangitsa kukhala chisankho chodziwika bwino pamayesero ambiri a biochemical.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
4-Nitrophenyl α-D-maltohexaoside ndi gulu la kalasi ya α-glycosidic linkages.Ndiwochokera ku maltose, yomwe ndi disaccharide yopangidwa ndi mayunitsi awiri a glucose.Pagululi, gulu la hydroxyl la gawo loyamba la shuga limalowetsedwa ndi nitrophenyl moiety.
Pagululi nthawi zambiri amagwiritsidwa ntchito ngati gawo laling'ono poyesa ma enzyme kuti aphunzire momwe ma enzymes osiyanasiyana amagwirira ntchito, makamaka omwe amakhudzidwa ndi metabolism ya carbohydrate.Gulu la nitrophenyl limalola kuzindikirika kosavuta komanso kuchuluka kwa machitidwe a enzymatic poyesa kuyamwa kapena fluorescence ya chinthu chong'ambika.
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PIPES CAS: 5625-37-6 Mtengo Wopanga
PIPES (piperazine-1,4-bisethanesulfonic acid) ndi zwitterionic buffering compound yomwe imagwiritsidwa ntchito pofufuza zamoyo ndi biochemical.Ndi pH buffer yogwira ntchito yokhala ndi mphamvu yayikulu yosunga pH yokhazikika mumtundu wa pH wa 6.1 mpaka 7.5.PIPES ili ndi zosokoneza pang'ono ndi ma biomolecules ndipo ndiyoyenera kuyesa kutengera kutentha.Nthawi zambiri amagwiritsidwa ntchito mu njira za gel electrophoresis ndi kupanga mankhwala monga wothandizira wokhazikika.Ponseponse, PIPES ndi gulu losunthika komanso logwiritsidwa ntchito kwambiri pamayesero osiyanasiyana.
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3,3′,5,5'-Tetramethylbenzidine CAS:207738-08-7
3,3',5,5'-Tetramethylbenzidine, yomwe imadziwikanso kuti TMB, ndi mankhwala omwe amagwiritsidwa ntchito ngati gawo lapansi la chromogenic mu ma enzyme-linked immunosorbent assays (ELISA) ndi mayeso ena a biochemical.Nthawi zambiri amagwiritsidwa ntchito kuti azindikire ndikuwerengera kukhalapo kwa michere monga horseradish peroxidase (HRP) m'matsampu osiyanasiyana achilengedwe.TMB imasintha mtundu kuchoka ku mtundu wopanda mtundu kukhala wabuluu pamaso pa ma enzymes awa.Pambuyo pake, zomwe zimachitikazo zitha kuyimitsidwa powonjezera asidi omwe amasintha mtundu wabuluu kukhala mtundu womaliza wachikasu.Kuchuluka kwa mtundu wachikasu kumayenderana ndi kuchuluka kwa ma enzyme omwe alipo, zomwe zimapangitsa kuti pakhale kuchuluka.
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APS-5 CAS:193884-53-6 Mtengo Wopanga
(4-Chlorophenyl) thio-methanol 1- (dihydrogen phosphate) mchere wa disodium (1: 2) ndi mankhwala omwe ali m'gulu la acridine derivatives.Amakhala ndi 10-methylacridine ring system yokhala ndi gulu la thioether lomwe limalumikizidwa ndi malo a 4-chlorophenyl.Pagululi limakhalanso ndi gulu la methanol ndi magulu awiri a phosphate omwe amasinthidwa pang'ono ndi ayoni a sodium.
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5-Bromo-4-chloro-3-indolyl phosphate disodium mchere CAS:102185-33-1
5-Bromo-4-chloro-3-indolyl phosphate disodium mchere (BCIP) ndi mankhwala omwe amagwiritsidwa ntchito kwambiri mu biology ndi biochemistry applications.Ndi gawo lapansi la chromogenic la michere ya alkaline phosphatase.
BCIP nthawi zambiri imagwiritsidwa ntchito limodzi ndi nitroblue tetrazolium (NBT) ngati gawo lapansi pozindikira zochita za alkaline phosphatase.Pamene BCIP ndi dephosphorylated ndi alkaline phosphatase, buluu precipitate mitundu, kulola kuwonetsera kukhalapo kapena ntchito enzyme.
Pagululi ndi lothandiza kwambiri pakugwiritsa ntchito monga immunohistochemistry, in situ hybridization, ndi ma enzyme-linked immunosorbent assays (ELISAs) kuti azindikire kupezeka kapena kumasulira kwa ma biomolecules kapena nucleic acid.Mpweya wa buluu wopangidwa ndi BCIP umapereka chizindikiro chowonekera chomwe chimathandiza kuzindikira ndi kusanthula mamolekyu omwe akutsata mu zitsanzo zoyesera.
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Mchere wamchere wa Sodium CAS: 139-41-3 Mtengo Wopanga
N,N-Bis(2-hydroxyethyl) glycine sodium mchere ndi mankhwala ntchito ngati buffering agent mu zosiyanasiyana biochemical ndi biophysical ntchito.Zimathandizira kukhala ndi pH yokhazikika pamayesero, ndikupangitsa kuti ikhale yothandiza mu maphunziro a enzyme, kafukufuku wamapuloteni, chikhalidwe cha ma cell, ndi njira zotsekera zaku Western.
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4-Aminophthalhydrazide AMPPD CAS: 3682-14-2
4-Aminophthalhydrazide, yomwe imadziwikanso kuti 4-APhH, ndi mankhwala omwe ali ndi mamolekyu a C8H8N2O.Ndi m'gulu la mankhwala a hydrazide ndipo amachokera ku phthalic acid.
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N-Acetyl-L-cysteine CAS: 616-91-1
N-Acetyl-L-cysteine (NAC) ndi mawonekedwe osinthidwa a amino acid cysteine.Amapereka gwero la cysteine ndipo amatha kusinthidwa mosavuta kukhala tripeptide glutathione, antioxidant wamphamvu m'thupi.NAC imadziwika chifukwa cha antioxidant ndi mucolytic properties, zomwe zimapangitsa kuti zikhale zothandiza pazinthu zosiyanasiyana zaumoyo.
Monga antioxidant, NAC imateteza maselo kuti asawonongeke chifukwa cha ma radicals aulere, mitundu ya okosijeni yokhazikika, ndi poizoni.Imathandiziranso kaphatikizidwe ka glutathione, komwe kumagwira ntchito yofunika kwambiri pakuchotsa poizoni m'thupi ndikusunga chitetezo chokwanira.
NAC yaphunziridwa chifukwa cha zopindulitsa zake paumoyo wa kupuma, makamaka kwa anthu omwe ali ndi matenda monga bronchitis, COPD, ndi cystic fibrosis.Nthawi zambiri amagwiritsidwa ntchito ngati expectorant kuti athandizire kuonda komanso kumasula ntchofu, zomwe zimapangitsa kuti zikhale zosavuta kuchotsa mpweya.
Kuphatikiza apo, NAC yawonetsa lonjezo lothandizira thanzi lachiwindi pothandizira kuchotsa zinthu zapoizoni, monga acetaminophen, chothandizira kupweteka wamba.Zitha kukhalanso ndi zoteteza ku chiwindi kuwonongeka chifukwa chakumwa mowa.
Kuphatikiza pa mphamvu zake zoteteza antioxidant ndi kupuma, NAC yafufuzidwa chifukwa cha zopindulitsa zake m'maganizo.Kafukufuku wina akuwonetsa kuti zitha kukhala ndi zotsatira zabwino pazovuta zamalingaliro, monga kupsinjika maganizo ndi matenda okakamiza (OCD).
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Acetylthiocholine iodide CAS: 1866-15-5
Acetylthiocholine iodide ndi mankhwala omwe amagwiritsidwa ntchito kwambiri ngati gawo laling'ono poyesa ma enzyme kuti ayese ntchito ya enzyme acetylcholinesterase (AChE).AChE ndi puloteni yomwe imayendetsa hydrolyzene neurotransmitter acetylcholine, sitepe yofunika kwambiri pothetsa kufalikira kwa chizindikiro pakati pa maselo a mitsempha.
Pamene iodide ya acetylthiocholine ikugwiritsidwa ntchito ndi AChE, gulu la acetyl limachotsedwa, zomwe zimapangitsa kupanga thiocholine ndi acetate ions.Thiocholine kenako amachitira ndi reagent yopanda mtundu yotchedwa DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) kuti ipange gulu lamtundu wachikasu lotchedwa 5-thio-2-nitrobenzoate, lomwe lingayesedwe mowonera.Mlingo wa kukula kwa mtundu umagwirizana mwachindunji ndi ntchito ya AChE pachitsanzo.
