5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) ndi mankhwala omwe amagwiritsidwa ntchito kwambiri mu biology ya mamolekyulu ndi biochemistry. Ndi gawo la chromogenic la ma enzymes a alkaline phosphatase.

BCIP nthawi zambiri imagwiritsidwa ntchito limodzi ndi nitroblue tetrazolium (NBT) ngati substrate kuti izindikire momwe alkaline phosphatase imagwirira ntchito. BCIP ikachotsedwa phosphorylated ndi alkaline phosphatase, buluu limayamba kupanga, zomwe zimathandiza kuti enzyme ione ngati ili kapena ikugwira ntchito.

Kapangidwe kameneka ndi kothandiza kwambiri pa ntchito monga immunohistochemistry, in situ hybridization, ndi enzyme-linked immunosorbent assays (ELISAs) kuti azindikire kukhalapo kapena malo a ma biomolecules enaake kapena ma nucleic acid. Mpweya wabuluu wopangidwa ndi BCIP umapereka chizindikiro chowoneka chomwe chimathandiza kuzindikira ndi kusanthula mamolekyu omwe akufunidwa mu zitsanzo zoyesera.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Immunohistochemistry (IHC): BCIP nthawi zambiri imagwiritsidwa ntchito poyesa IHC kuti iwonetse kukhalapo kapena malo a ma antigen enaake m'zitsanzo za minofu. Kuchuluka kwa buluu komwe kumapangidwa ndi BCIP kumalola kuzindikira malo a antigenic ndipo kungagwiritsidwe ntchito poyesa qualitative kapena semi-quantitative.

Kusakaniza kwa malo (ISH): BCIP imagwiritsidwa ntchito kwambiri mu njira za ISH kuti izindikire ndikuzindikira ma nucleic acid sequences enaake, monga mRNA kapena DNA. Kuthamanga kwa buluu komwe kumapangidwa ndi BCIP kumasonyeza kukhalapo kwa ma target sequences ndipo kumathandiza kuwona momwe amafalikira mkati mwa maselo kapena minofu.

Kuyesa kwa immunosorbent komwe kumalumikizidwa ndi enzyme (ELISAs): Nthawi zina BCIP imagwiritsidwa ntchito mu ELISA kuti izindikire kukhalapo kapena kuchuluka kwa ma biomolecule enaake, monga mapuloteni kapena ma antibodies. Mwa kuphatikiza BCIP ndi chizindikiro choyenera cha enzyme ndikugwiritsa ntchito alkaline phosphatase kuti ipangitse kuti zinthu ziyambe kuyenda bwino, mtundu wabuluu womwe umapangidwa ungasonyeze kukhalapo ndi kuchuluka kwa biomolecule yomwe mukufuna.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C8H7BrClNNaO4P
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	102185-33-1
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.