Fluorescein Isothiocyante CAS:3326-32-7

Fluorescein isothiocyanate (FITC) ndi utoto wa fluorescent womwe umagwiritsidwa ntchito kwambiri mu kafukufuku wa zamankhwala ndi ntchito zowunikira. Ndi mtundu wa fluorescein womwe wasinthidwa kuti ukhale ndi gulu la isothiocyanate, lomwe limalola kuti ligwirizane ndi mapuloteni, ma antibodies, ndi ma biomolecule ena.

FITC imatulutsa kuwala kobiriwira kowala ikakhudzidwa ndi kuwala kwa kutalika kwa nthawi yoyenera, nthawi zambiri pafupifupi 488 nm. Kuwala kumeneku kumatha kuzindikirika ndikuyesedwa pogwiritsa ntchito njira zosiyanasiyana zojambulira zithunzi, monga fluorescence microscopy, flow cytometry, ndi fluorescence spectroscopy.

Mwa kuphatikiza FITC ndi ma biomolecule ofunikira, ofufuza amatha kutsatira ndikuwona momwe ma biomolecule awa amagawidwira, malo ake, ndi momwe amagwirira ntchito mkati mwa maselo, minofu, kapena zitsanzo zamoyo. Mwachitsanzo, ma antibodies okhala ndi chizindikiro cha FITC angagwiritsidwe ntchito kuzindikira mapuloteni kapena ma antigen enaake mu mayeso a immunofluorescence kapena kusanthula kuchuluka kwa maselo mu kuyesa kwa flow cytometry.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kapangidwe ka kuwala: FITC ili ndi kuchuluka kwakukulu kwa quantum ndipo imatulutsa kuwala kobiriwira kowala ikayamba kugwedezeka pafupifupi 488 nm. Kapangidwe kake ka kuwala kamapangitsa kuti ikhale yoyenera kuzindikirika pogwiritsa ntchito njira zosiyanasiyana zojambulira, monga fluorescence microscopy, flow cytometry, ndi fluorescence spectroscopy.

Kumangirirana kwa Covalent: FITC ili ndi gulu la isothiocyanate lomwe limalola kuti ligwirizane ndi magulu a amino omwe ali m'mapuloteni, ma antibodies, ndi ma biomolecule ena. Kumangirirana kwa covalent kumeneku kumatsimikizira kulemba zilembo zokhazikika komanso zenizeni za mamolekyu omwe akufuna, zomwe zimathandiza kuzindikira ndi kuwonetsa.

Kuunikira kwa Chitetezo cha Mthupi: Ma antibodies okhala ndi chizindikiro cha FITC amagwiritsidwa ntchito kwambiri poyesa ma immunofluorescence kuti azindikire ma antigen kapena mapuloteni enaake m'maselo ndi minofu. Kuwala kobiriwira kowala komwe kumatulutsidwa ndi FITC kumathandiza ofufuza kupeza ndikuwona momwe mamolekyu omwe akufuna kufalikira mkati mwa zitsanzo zamoyo amagawidwira.

Flow cytometry: Ma antibodies olembedwa ndi FITC kapena ma biomolecule ena olumikizidwa ndi fluorochrome amagwiritsidwa ntchito kwambiri mu flow cytometry kuti afufuze ndikusankha maselo kutengera zizindikiro zawo zapamwamba kapena zigawo zamkati mwa maselo. FITC imagwirizana ndi laser ya 488 nm yomwe imagwiritsidwa ntchito kwambiri mu zida za flow cytometry.

Maphunziro okhudza kuyanjana kwa mapuloteni: FITC ingagwiritsidwe ntchito mu maphunziro okhudza kuyanjana kwa mapuloteni ndi mapuloteni kuti aone momwe mapuloteni amamangirirana komanso momwe mapuloteni amagwirira ntchito. Mwa kulemba ma protein enaake ndi FITC, ofufuza amatha kusanthula kuyanjana kwawo pogwiritsa ntchito njira monga fluorescence resonance energy transfer (FRET) kapena fluorescence polarization.

Kutsata mamolekyulu a biomolecule: FITC ingagwiritsidwe ntchito kutsatira ndikutsatira mayendedwe a mamolekyulu a biomolecule mkati mwa maselo amoyo kapena minofu. Mwa kulemba mamolekyulu ofunikira ndi FITC, ofufuza amatha kuphunzira malo awo, mphamvu zawo, ndi machitidwe awo ogulitsa nthawi yeniyeni pogwiritsa ntchito microscopy ya fluorescence.

Kugwiritsa Ntchito Matenda: Ma probe kapena ma antibodies okhala ndi chizindikiro cha FITC amagwiritsidwa ntchito pofufuza matenda osiyanasiyana, kuphatikizapo kuzindikira ma antigen enaake, zinthu zopatsirana, kapena ma biomarker m'zitsanzo za odwala. Ma probe amenewa amapereka kuzindikira kwapadera komanso kwapadera, zomwe zimathandiza kuzindikira matenda mwachangu komanso molondola.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C21H11NO5S
Kuyesa	99%
Maonekedwe	Ufa wachikasu
Nambala ya CAS	3326-32-7
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.