FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside, yomwe imadziwikanso kuti FMG, ndi mankhwala opangidwa ndi kuwala omwe amagwiritsidwa ntchito kwambiri ngati gawo la zinthu m'mayesero osiyanasiyana a biochemical ndi cell biology. Amachokera ku methyl-beta-D-galactopyranoside powagwirizanitsa ndi molekyulu ya fluorescein.FMG imagwiritsidwa ntchito kwambiri pophunzira ntchito ya beta-galactosidase, enzyme yomwe imayambitsa hydrolysis ya lactose kukhala galactose ndi glucose. Pogwiritsa ntchito FMG ngati gawo la zinthu, ofufuza amatha kuyang'anira ntchito ya enzyme ya beta-galactosidase poyesa kutulutsa kwa fluorescence. Hydrolysis ya FMG ndi beta-galactosidase imabweretsa kutulutsidwa kwa fluorescein, zomwe zimapangitsa kuti chizindikiro cha fluorescent chiwonjezeke chomwe chingayesedwe. Mankhwalawa amagwiritsidwanso ntchito pofufuza kuzindikira ndi kuyanjana kwa chakudya. FMG ingagwiritsidwe ntchito ngati chofufuzira cha mamolekyu kuti iphunzire mgwirizano womangirira wa lectins (mapuloteni omwe amamangirira makamaka ku chakudya) ku chakudya chokhala ndi galactose. Kumangirira kwa ma FMG-lectin complexes kumatha kuzindikirika ndikuyesedwa kutengera kusintha kwa kutulutsa kwa fluorescence. Ponseponse, FMG ndi chida chogwiritsidwa ntchito kwambiri pophunzira ntchito ya ma enzyme ndi kuzindikira chakudya, zomwe zimapereka njira yosavuta komanso yodziwira bwino yoyezera fluorescence ndikuwunika njira zamoyozi.

Oda kapena Pemphani Mtengo

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Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Fluorescein mono-beta-D-galactopyranoside (FMG) ndi molekyulu yomwe imagwiritsidwa ntchito kwambiri mu kafukufuku wa zamoyo ngati gawo lothandizira kuzindikira kukhalapo ndi ntchito ya enzyme ya beta-galactosidase. FMG ndi yochokera ku shuga wa lactose ndipo imalumikizidwa ndi molekyulu ya fluorescein.

Chotsatira chachikulu cha FMG ndichakuti imasungunuka ndi hydrolyzed ndi beta-galactosidase, enzyme yomwe imaphwanya lactose kukhala galactose ndi shuga. Kusungunuka kwa enzyme kumeneku kwa FMG kumapangitsa kuti fluorescein itulutsidwe, yomwe imatulutsa chizindikiro champhamvu cha fluorescence.

Kugwiritsa ntchito kwakukulu kwa FMG ndiko kuzindikira ndi kuyeza ntchito ya beta-galactosidase m'masampulo osiyanasiyana. Enzyme iyi imapezeka m'zamoyo zambiri, kuphatikizapo mabakiteriya ndi maselo a zinyama zoyamwitsa, ndipo ntchito yake ikhoza kusonyeza njira zosiyanasiyana zamaselo ndi njira za kagayidwe kachakudya.

Pogwiritsa ntchito FMG ngati substrate, ntchito ya beta-galactosidase imatha kuyezedwa poyang'anira kuwala komwe kumatulutsa ndi fluorescein yotulutsidwa. Kuyeza kumeneku kungachitike m'mayesero osiyanasiyana, kuphatikizapo kuyesa kwa in vitro ndi maphunziro ojambula maselo amoyo.

Kuphatikiza apo, FMG ingagwiritsidwe ntchito ngati chida chophunzirira kufalikira ndi malo a beta-galactosidase mkati mwa maselo. Pogwiritsa ntchito njira zowunikira ma microscopy, ofufuza amatha kuwona kuwala komwe kumachokera ku FMG panthawi ya hydrolysis, zomwe zimawathandiza kutsatira momwe beta-galactosidase imagwirira ntchito m'malo ndi nthawi.

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Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C26H22O10
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	102286-67-9
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.