HEPBS CAS:161308-36-7 Mtengo wa Wopanga

N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid) (HEPBS) ndi zwitterionic buffer yomwe imagwiritsidwa ntchito kwambiri mu kafukufuku wa zamoyo ndi zamankhwala. Chotsatira chake chachikulu ndikuthandiza kusunga pH yokhazikika mu mayankho, makamaka mkati mwa pH ya thupi (7.2-7.4).

Kugwiritsa ntchito kwakukulu kwaHEPBS Ili mu cell culture, komwe imagwiritsidwa ntchito ngati gawo la culture media kuti isunge pH ya yankho. Imathandiza kupereka malo okhazikika oti maselo akule komanso kupewa kusintha kulikonse kwa pH komwe kungawononge maselo.

HEPBS imagwiritsidwanso ntchito ngati chotetezera mu maphunziro a ma enzyme, chifukwa imatha kukhazikika pH panthawi ya ma enzyme. Nthawi zambiri imagwiritsidwa ntchito poyeretsa mapuloteni ndi ma enzyme kuti iwonjezere ntchito ndi kukhazikika kwa ma enzyme.

Komanso,HEPBS imagwiritsidwa ntchito m'njira zosiyanasiyana za electrophoretic, monga gel electrophoresis ndi capillary electrophoresis, kuti isunge pH yofunikira ndikukhazikitsa mamolekyulu omwe akulekanitsidwa.

Kuwonjezera pa makhalidwe ake otetezera,HEPBS ingathenso kugwira ntchito ngati choletsa chofooka cha ma metalloprotein ndi ma enzyme ena, zomwe zimapangitsa kuti ikhale yothandiza pa ntchito zinazake.