HEPES-Na CAS:75277-39-3 Mtengo Wopanga

Buffering agent: Mchere wa sodium wa HEPES umagwiritsidwa ntchito nthawi zambiri ngati chosungira poyesa zamoyo ndi zamankhwala.Zimathandizira kukhala ndi pH yokhazikika, makamaka mumtundu wa thupi (pH 7.2-7.6).Kuchulukitsa kwake kumapangitsa kukhala kofunikira pakusunga mikhalidwe yoyenera pamachitidwe osiyanasiyana a enzymatic, chikhalidwe cha ma cell, ndi njira zama cell biology.

Chikhalidwe cha ma cell: Mchere wa HEPES wa sodium umagwiritsidwa ntchito kwambiri ngati chotchinga mu media media.Kutha kusunga pH yokhazikika ndikofunikira kuti ma cell akule bwino komanso kuti azikhala olimba.HEPES nthawi zambiri imakondedwa kuposa ma buffering agents chifukwa sichiwonetsa kusintha kwakukulu mu pH ikakumana ndi CO2 ya mumlengalenga.

Maphunziro a enzyme: Mchere wa sodium wa HEPES ndiwothandiza makamaka m'maphunziro a enzymatic pomwe malo okhazikika a pH amafunikira.Zimalepheretsa kusinthasintha kwakukulu kwa pH panthawi ya zochitika za enzymatic, kuonetsetsa kuti ma enzyme akugwira ntchito bwino.

Electrophoresis: Mchere wa HEPES wa sodium umagwiritsidwa ntchito ngati chosungira munjira zosiyanasiyana zama electrophoretic monga polyacrylamide gel electrophoresis (PAGE) ndi agarose gel electrophoresis.Imathandiza kusunga pH ya nkhokwe, yomwe ndiyofunikira pakulekanitsa ndi kusanthula ma nucleic acid ndi mapuloteni.

Mayeso a biochemical: Mchere wa HEPES wa sodium umagwiritsidwa ntchito nthawi zambiri pakuyesa kwa biochemical, kuphatikiza kuyesa kwa ma enzyme, kuyesa kwa protein quantification, ndi kuyesa kwa spectrophotometric.Zimathandizira kukhalabe ndi pH yomwe mukufuna kuti mupeze zotsatira zolondola komanso zobwerezabwereza.

Mapangidwe a mankhwala: Mchere wa HEPES wa sodium umagwiritsidwanso ntchito m'mapangidwe amankhwala ngati njira yochepetsera kukhazikika kwa mankhwala ndikusunga pH yofunikira.