P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

P-Nitrophenyl beta-D-lactopyranoside, yomwe imadziwikanso kuti PNPG, ndi mankhwala omwe amagwiritsidwa ntchito nthawi zambiri poyesa ma enzyme kuti ayesere ntchito ya beta-galactosidase, enzyme yomwe imakhudzidwa ndi kagayidwe ka chakudya m'thupi. PNPG ndi chinthu chopangidwa chomwe chingadulidwe ndi beta-galactosidase, zomwe zimapangitsa kuti chinthucho chitulutsidwe chachikasu. Kukula kwa hydrolysis ya substrate kumatha kuwerengedwa ndi spectrophotometric poyesa kuyamwa kwa chinthucho pamlingo winawake. Izi zimathandiza ofufuza kuwunika ntchito ndi kinetics ya beta-galactosidase m'malo osiyanasiyana, monga kuphunzira ntchito ya enzyme, kufufuza zoletsa za enzyme kapena zoyambitsa, kapena kuwunika zotsatira za kusintha kwa masinthidwe pa ntchito ya enzyme.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kuzindikira ntchito ya beta-galactosidase: PNPG imagwiritsidwa ntchito kwambiri poyesa ntchito ya beta-galactosidase, enzyme yomwe imayambitsa hydrolysis ya lactose kukhala shuga ndi galactose. Hydrolysis ya PNPG ndi beta-galactosidase imatulutsa molekyulu ya p-nitrophenol (pNP), yomwe imatha kuzindikirika ndi spectrophotometric chifukwa cha mtundu wake wachikasu.

Kufufuza zinthu zoletsa ma enzyme ndi zoyambitsa: PNPG ingagwiritsidwe ntchito pofufuza zinthu zomwe zimapangitsa kuti pakhale mphamvu zambiri kuti izindikire zinthu zomwe zimasinthasintha zochita za beta-galactosidase. Poyesa kuchuluka kwa PNPG hydrolysis pamaso pa zinthu zosiyanasiyana zoyesera, ofufuza amatha kuzindikira zinthu zomwe zimachepetsa ntchito ya ma enzyme kapena zinthu zomwe zimapangitsa kuti ma enzyme azigwira ntchito bwino.

Kuphunzira za kinetics ya enzyme: Kuchuluka kwa hydrolysis ya PNPG ndi beta-galactosidase kumatsatira kinetics ya Michaelis-Menten, zomwe zimathandiza ofufuza kudziwa magawo ofunikira a enzyme monga liwiro lalikulu la reaction (Vmax) ndi Michaelis constant (Km). Izi zimathandiza kumvetsetsa kuyanjana kwa substrate ya enzyme ndi mphamvu yake yothandiza.

Kugwiritsa ntchito kwa biology ya mamolekyulu: Beta-galactosidase, yomwe imadula PNPG, imagwiritsidwa ntchito kwambiri ngati jini yolembera nkhani mu biology ya mamolekyulu. Gawo la PNPG nthawi zambiri limagwiritsidwa ntchito kuzindikira ndikuwona momwe jini yolembera nkhani imaonekera, zomwe zimapereka njira yosavuta komanso yodziwira momwe jini imaonekera m'machitidwe osiyanasiyana oyesera.

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Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C18H25NO13
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	4419-94-7
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.