N-Ethylmaleimide CAS:128-53-0 Mtengo wa Wopanga

N-Ethylmaleimide (NEM) ndi mankhwala ang'onoang'ono achilengedwe omwe amagwiritsidwa ntchito kwambiri mu kafukufuku wa biochemistry ndi molecular biology. Imagwira ntchito ngati choletsa chapadera cha magulu a protein sulfhydryl (thiol) mwa kusintha mosasinthika ndikuletsa ntchito yawo. NEM imagwira ntchito kwambiri ndi magulu a sulfhydryl, monga omwe amapezeka mu amino acid cysteine, ndipo imatha kuchitapo kanthu ndi magulu a free sulfhydryl komanso omwe ali mkati mwa mapuloteni. Izi zimapangitsa NEM kukhala chida chothandiza pophunzira ntchito ya mapuloteni, kuyanjana kwa mapuloteni ndi mapuloteni, ndi ntchito ya enzyme. Makhalidwe ake oletsa agwiritsidwa ntchito m'njira zosiyanasiyana, kuphatikizapo proteomics, enzymology, structural biology, ndi kupeza mankhwala.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kusintha kwa Mapuloteni: NEM imasintha mosasinthika zotsalira za cysteine m'mapuloteni mwa kupanga covalent adduct. Kusintha kumeneku kungakhudze ntchito ya mapuloteni, kapangidwe kake, ndi kuyanjana kwake. Kuchita kwa NEM ndi magulu a sulfhydryl kumapangitsa kuti ikhale chida chofunikira pophunzira ntchito ya mapuloteni ndi njira zomwe zimadalira thiol.

Kuletsa Ma Enzyme: Ma Enzyme ambiri amadalira magulu a thiol kuti agwire ntchito yawo yothandiza. Mwa kuchitapo kanthu ndi magulu a thiol, NEM imatha kuletsa ma enzyme enaake mwachisawawa. Izi zagwiritsidwa ntchito kwambiri pophunzira momwe ma enzyme amagwirira ntchito, kumvetsetsa njira za ma enzyme, ndi kuyesa zoletsa ma enzyme zomwe zingatheke.

Kafukufuku Wokhudza Kuyanjana kwa Mapuloteni ndi Mapuloteni: NEM nthawi zambiri imagwiritsidwa ntchito pofufuza momwe mapuloteni amagwirira ntchito. Mwa kuletsa mosasamala zotsalira za cysteine, NEM imaletsa kupangika kwa ma disulfide bond pakati pa mapuloteni ogwirizana, motero kusokoneza kuyanjana kwawo. Izi zimathandiza ofufuza kuphunzira ntchito ya ma cysteine enaake pa kuyanjana kwa mapuloteni ndi mapuloteni.

Maphunziro a Redox Regulation: NEM imagwiritsidwa ntchito pophunzira njira zoyendetsera redox m'mapuloteni. Mwa kusintha ma cysteine osinthika, NEM imatha kukhazikika pamapangidwe enaake a mapuloteni ndikuletsa okosijeni kapena kuchepa kwa zotsalira za cysteine. Izi zimathandiza kumvetsetsa ntchito ya redox signaling ndi njira zowongolera zomwe zimakhudzidwa ndi redox.

Maphunziro Ogwirizanitsa Mapuloteni: NEM ingagwiritsidwe ntchito ngati cholumikizira chogwirizanitsa kuti ikhazikitse mapuloteni kapena kuyanjana kwa mapuloteni ndi mapuloteni. Mwa kusintha zotsalira za cysteine, NEM ikhoza kupanga mgwirizano wogwirizana pakati pa mapuloteni ogwirizana, zomwe zimathandiza ofufuza kufufuza mapuloteni ogwirizana ndi kapangidwe kawo. Njirayi ndi yothandiza makamaka mu maphunziro a biology ndi proteomics.

Kupeza Mankhwala: Chifukwa cha kuthekera kwake kochita zinthu mosankha ndi zotsalira za cysteine, NEM yagwiritsidwa ntchito pofufuza za kupeza mankhwala. Itha kugwiritsidwa ntchito kuzindikira ndikufotokozera zomwe zingakhalepo pa mankhwala kapena kuphunzira za mankhwala enieni omwe angakhalepo pa mapuloteni olemera mu cysteine.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C6H7NO2
Kuyesa	99%
Maonekedwe	ufa woyera wa kristalo
Nambala ya CAS	128-53-0
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.