NBD chloride CAS: 10199-89-0 Chithunzi Chodziwika

NBD kloride CAS: 10199-89-0

NBD chloride, yomwe imadziwikanso kuti 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, ndi utoto wa fluorescent womwe umagwiritsidwa ntchito kwambiri mu mankhwala ndi zamoyo. Ndi wa gulu la zinthu zochokera ku nitrobenzoxadiazole (NBD), zomwe zimakhala ndi mphamvu zowala kwambiri.

NBD chloride imadziwika ndi kuthekera kwake kupanga ma covalent bond ndi ma nucleophiles, monga ma primary amines, ma thiols, ndi magulu a hydroxyl. Kapangidwe kameneka kamalola kuti kagwiritsidwe ntchito ngati cholembera ma biomolecules, kuphatikizapo mapuloteni, ma peptides, ma nucleic acids, ndi mamolekyu ena ang'onoang'ono.

Pamene NBD chloride ichitapo kanthu ndi nucleophile, imakumana ndi kusintha kwa zinthu, zomwe zimapangitsa kuti pakhale chinthu chokhazikika chokhala ndi chizindikiro cha NBD. Chinthu cholembedwachi chimasonyeza kutulutsa kwamphamvu kwa fluorescence, zomwe zimapangitsa kuti chikhale chothandiza pa njira zosiyanasiyana zozindikira ndi kujambula.

Kutuluka kwa kuwala kwa mamolekyulu okhala ndi zilembo za NBD kumatha kusinthidwa ndi kuwala kwa ultraviolet (UV) pafupifupi 470 nm, ndipo kuwala komwe kumatulutsidwa nthawi zambiri kumadziwika pafupifupi 530 nm. Kutalika kwa kuwala kumeneku kumagwera m'dera lobiriwira la sipekitiramu yowoneka, komwe kusokoneza kuzindikira kuchokera ku zitsanzo zamoyo kumakhala kochepa.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kulemba zilembo za Covalent: NBD chloride imatha kupanga ma covalent bond ndi magulu a nucleophilic m'magulu a biomolecules, monga ma primary amines, thiols, ndi magulu a hydroxyl. Izi zimathandiza kulemba zilembo za biomolecules izi kuti zidziwike ndi kujambula.

Kuwonjezeka kwa kuwala: Pamene NBD chloride imagwirizana ndi ma nucleophiles, imapanga chinthu cholembedwa ndi NBD chowala. Chinthu cholembedwachi chimasonyeza kutulutsa kwamphamvu kwa kuwala, zomwe zimapangitsa kuti chikhale chothandiza powonetsa ndikutsatira mamolekyu olembedwa.

Kuzindikira ndi kutsimikiza: Kutuluka kwa kuwala kwa zinthu zolembedwa ndi NBD kumakhala kovuta kwambiri, zomwe zimapangitsa kuti mamolekyu ochepa olembedwa akhale ochepa. Kuphatikiza apo, kupangika kwa mgwirizano wa covalent pakati pa NBD chloride ndi ma nucleophiles kumapereka kutsimikizika, chifukwa kumatsimikizira kuti utotowo umakhala wolimba komanso wapadera ku biomolecule yomwe mukufuna.

Zolemba za biomolecule: NBD chloride ingagwiritsidwe ntchito kulemba zilembo za biomolecules zosiyanasiyana, kuphatikizapo mapuloteni, ma peptides, nucleic acids, ndi mamolekyu ena ang'onoang'ono. Izi zimathandiza ofufuza kufufuza malo, mphamvu, ndi momwe ma biomolecules awa amagwirira ntchito m'machitidwe a zamoyo.

Kujambula kwa maikulosikopu ya kuwala: Ma biomolecule okhala ndi chizindikiro cha NBD amatha kuonedwa pogwiritsa ntchito maikulosikopu ya kuwala. Njira imeneyi imalola kuwona mamolekyu olembedwa chizindikiro pamlingo wa selo kapena wa subcellular, zomwe zimapereka chidziwitso chofunikira chokhudza kufalikira kwawo ndi khalidwe lawo.

Kuchuluka kwa ma flow cytometry: Ma biomolecule okhala ndi zilembo za NBD amatha kufufuzidwa ndikuyesedwa pogwiritsa ntchito flow cytometry. Njirayi imalola kusanthula kwakukulu kwa maselo kapena tinthu tating'onoting'ono tomwe timalembedwa, kupereka chidziwitso chokhudza kuchuluka kwa mafotokozedwe kapena mawonekedwe a pamwamba pa ma biomolecule olembedwa.

Kulemba ndi kutsatira mapuloteni: NBD chloride ingagwiritsidwe ntchito kulemba ma amino acid otsala kapena magulu ogwira ntchito m'mapuloteni. Izi zimathandiza ofufuza kuphunzira kapangidwe ka mapuloteni, kupindika, mphamvu, ndi kuyanjana kwake nthawi yeniyeni.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C6H2ClN3O3
Kuyesa	99%
Maonekedwe	Ufa wachikasu
Nambala ya CAS	10199-89-0
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.