Dithiothreitol (DTT), CAS: 3483-12-3 mtundu watsopano wa zowonjezera zobiriwira

Dithiothreitol (DTT), CAS: 3483-12-3, monga kafukufuku wa sayansi wogwiritsidwa ntchito kwambiri, nthawi zambiri amagwiritsidwa ntchito monga kuchepetsa DNA ya sulfhydryl, deprotecting agent, ndi kuchepetsa zomangira za disulfide mu mapuloteni.Mtundu watsopano wa zowonjezera zobiriwira zimagwira ntchito yofunikira pakuwongolera magwiridwe antchito a batri.

Dithiothreitol (DTT) ndi amphamvu kuchepetsa wothandizila, ndipo reducibility makamaka chifukwa conformational kukhazikika kwa mphete zisanu ndi chimodzi (yokhala disulfide bond) mu makutidwe ndi okosijeni.Kuchepetsa kwa chomangira cha disulfide ndi dithiothreitol kumakhala ndi machitidwe awiri otsatizana a sulfhydryl-disulfide bond exchange.Mphamvu yochepetsera ya dithiothreitol (DTT) imakhudzidwa ndi pH mtengo, ndipo imatha kuthandizira kuchepetsa pamene pH mtengo uli waukulu kuposa 7. Izi ndichifukwa choti ma thiolate anions okha omwe amachotsedwa amakhala okhudzidwa, pamene mercaptans satero, ndipo pKa yamagulu a mercapto nthawi zambiri imakhala 8.3.

Dithiothreitol (DTT) nthawi zambiri amagwiritsidwa ntchito kuchepetsa zomangira za disulfide zama protein molekyulu ndi ma polypeptides.Nthawi zambiri amagwiritsidwa ntchito ngati mapuloteni a sulfhydryl oteteza ndipo amagwiritsidwa ntchito pokonzekera katemera kuti ateteze zotsalira za cysteine ​​​​kupanga intramolecular ndi intermolecular disulfides.kiyi.Pakuzindikira kwa nucleic acid, dithiothreitol (DTT) imatha kuwononga zomangira za disulfide mu protein ya RNase, kuyika RNase, ndikuwongolera zoyeserera monga nyumba ya library ya RNA ndi kukulitsa kwa RNA.Dithiothreitol (DTT) imagwiritsidwanso ntchito ngati antidote kuteteza maselo ndi minofu, monga radioprotectant, etc.

Komabe, dithiothreitol (DTT) nthawi zambiri satha kuchepetsa zomangira za disulfide zomwe zimayikidwa mu dongosolo la mapuloteni (zosungunulira zosafikirika).Kuchepetsa kwa zomangira za disulfide zotere nthawi zambiri kumafuna kusinthidwa kwa mapuloteni poyamba.

Pofuna ziletsa shuttle zotsatira za mabatire lifiyamu-sulfure ndi kusintha electrochemical ntchito ya mabatire lifiyamu-sulfure, yesani kugwiritsa ntchito dithiothreitol (DTT) ngati wometa ubweya wothandizila kukameta ubweya wapamwamba-kuti polysulfides kuwaletsa Kutha.Threitol (DTT) amasakanizidwa mu multi-mipanda mpweya nanotubes (MWCNTs) pepala kukonza DTT interlayer.The DTT interlayer aikidwa pakati zabwino elekitirodi pepala ndi olekanitsa wa lifiyamu-sulfure batani theka-selo, ndi sulfure kunyamula pamwamba osalimba a zabwino elekitirodi pepala About 2mg/cm2.Zotsatira zakuwunika kwa SEM zimatsimikizira kuti DTT imamwazikana mofanana pamwamba ndi zotsalira za pepala la MWCNTs.Electrochemical test zotsatira zimasonyeza kuti lifiyamu-sulfure batire ndi DTT sangweji dongosolo ali woyamba kutulutsa mphamvu yeniyeni ya 1288 mAh/g pa mlingo wa 0.05C.Kwa nthawi yoyamba, mphamvu ya coulombic ili pafupi ndi 100%, ndipo mphamvu yeniyeni panthawi ya malipiro ndi kutulutsa pa 0.5C, 2C, ndi 4C mitengo imafika 650mAh / g, 600mAh / g, ndi 410mAh / g, motero.Kukhazikitsidwa kwa kapangidwe ka masangweji a DTT kumatha kumeta ma polysulfides apamwamba kwambiri.Zimalepheretsa kusamukira ku lithiamu negative electrode, potero kulepheretsa kusuntha kwa shuttle ndikuwongolera kukhazikika kwa mayendedwe ndi mphamvu ya coulomb ya mabatire a lithiamu-sulfure.

Ndizofunikira kudziwa kuti dithiothreitol (DTT) ndi chinthu chapoizoni.Mwachitsanzo, pamaso pa zitsulo zosinthika, dithiothreitol (DTT) imatha kuwononga mamolekyu achilengedwe.Nthawi yomweyo, dithiothreitol (DTT) ) Itha kukulitsanso kawopsedwe ka mankhwala ena okhala ndi arsenic ndi mercury.Dithiothreitol (DTT) ili ndi fungo lopweteka, lomwe lingakhale lovulaza thanzi chifukwa cha kupuma ndi kukhudzana ndi khungu.Chifukwa chake, ndikofunikira kuti muteteze panthawi yogwira ntchito, kuvala masks, magolovesi ndi magalasi, ndikugwira ntchito mu fume hood.