Dithiothreitol (DTT), CAS: 3483-12-3, monga reagent yofufuza zasayansi yomwe imagwiritsidwa ntchito kwambiri, nthawi zambiri imagwiritsidwa ntchito ngati reducer ya sulfhydryl DNA, wothandizira woletsa chitetezo, komanso kuchepetsa ma disulfide bond mu mapuloteni. Mtundu watsopano wa zowonjezera zobiriwira umagwira ntchito yofunika kwambiri pakukweza magwiridwe antchito a batri.
Dithiothreitol (DTT) ndi mankhwala amphamvu ochepetsa mphamvu, ndipo kuchepa kwake kumachitika makamaka chifukwa cha kukhazikika kwa mphete yokhala ndi ziwalo zisanu ndi chimodzi (yomwe ili ndi ma bond a disulfide) mu mkhalidwe wake wa okosijeni. Kuchepa kwa bond wamba wa disulfide ndi dithiothreitol kumakhala ndi machitidwe awiri otsatizana a sulfhydryl-disulfide bond exchange reactions. Mphamvu yochepetsera mphamvu ya dithiothreitol (DTT) imakhudzidwa ndi pH value, ndipo imatha kuchepetsa mphamvu pokhapokha pH value ili yoposa 7. Izi zili choncho chifukwa ma anion a deprotonated thiolate okha ndi omwe amayankha, pomwe ma mercaptan satero, ndipo pKa ya magulu a mercapto nthawi zambiri ndi 8.3.
Dithiothreitol (DTT) imagwiritsidwa ntchito kwambiri kuchepetsa ma disulfide bonds a ma protein molecules ndi ma polypeptides. Nthawi zambiri imagwiritsidwa ntchito ngati protective protein sulfhydryl agent ndipo imagwiritsidwa ntchito popanga katemera kuti iteteze protein cysteine zotsalira kuti zisapange intramolecular ndi intermolecular disulfides. Mu njira yodziwira nucleic acid, dithiothreitol (DTT) imatha kuwononga ma disulfide bonds mu RNase protein, kuwononga RNase, ndikuthandizira kuchita zoyeserera monga RNA laibulale building ndi RNA amplification. Dithiothreitol (DTT) imagwiritsidwanso ntchito ngati mankhwala oteteza maselo ndi minofu, monga radioprotective, ndi zina zotero.
Komabe, dithiothreitol (DTT) nthawi zambiri silingathe kuchepetsa ma disulfide bond omwe ali mu kapangidwe ka mapuloteni (zosungunulira sizingafikire). Kuchepetsa ma disulfide bond otere nthawi zambiri kumafuna kusintha kwa puloteni kaye.
Pofuna kuletsa mphamvu ya mabatire a lithiamu-sulfur yothamanga komanso kukonza magwiridwe antchito amagetsi a mabatire a lithiamu-sulfur, yesani kugwiritsa ntchito dithiothreitol (DTT) ngati chodulira kuti mudule ma polysulfide apamwamba kuti asasungunuke. Threitol (DTT) imasakanizidwa mu pepala la nanotubes la kaboni (MWCNTs) lomwe lili ndi makoma ambiri kuti lipange chodulira cha DTT. Chodulira cha DTT chimayikidwa pakati pa pepala la electrode labwino ndi cholekanitsa batani la lithiamu-sulfur theka la selo, ndi kuchuluka kwa pamwamba pa pepala la electrode labwino pafupifupi 2mg/cm2. Zotsatira za SEM zowonera zimatsimikizira kuti DTT imafalikira mofanana pamwamba ndi m'malo opanda kanthu a pepala la MWCNTs. Zotsatira za mayeso a electrochemical zikuwonetsa kuti batire ya lithiamu-sulfur yokhala ndi kapangidwe ka sandwich ya DTT ili ndi mphamvu yoyamba yotulutsa ya 1288 mAh/g pamlingo wa 0.05C. Kwa nthawi yoyamba, mphamvu ya coulombic ili pafupi ndi 100%, ndipo mphamvu yeniyeni panthawi yolipiritsa ndi kutulutsa mphamvu pa 0.5C, 2C, ndi 4C imafika pa 650mAh/g, 600mAh/g, ndi 410mAh/g, motsatana. Kuyamba kwa kapangidwe ka sandwich ya DTT kumatha kumeta bwino ma polysulfide apamwamba. Kumalepheretsa kusamuka kupita ku lithiamu negative electrode, motero kumaletsa mphamvu ya shuttle ndikukweza kukhazikika kwa kayendedwe ka madzi ndi mphamvu ya coulomb ya mabatire a lithiamu-sulfur.
Ndikofunika kudziwa kuti dithiothreitol (DTT) ndi mankhwala oopsa. Mwachitsanzo, ngati pali zitsulo zosinthika, dithiothreitol (DTT) imatha kuwononga mamolekyu achilengedwe. Nthawi yomweyo, dithiothreitol (DTT) imathanso kuwonjezera poizoni wa mankhwala ena okhala ndi arsenic ndi mercury. Dithiothreitol (DTT) ili ndi fungo loipa, lomwe lingakhale lovulaza thanzi chifukwa chopuma mpweya komanso kukhudzana ndi khungu. Chifukwa chake, ndikofunikira kuiteteza ikagwiritsidwa ntchito, kuvala masks, magolovesi ndi magalasi, ndikugwiritsa ntchito mu chivundikiro cha fume.
Thithreitol (DTT) ngati chometa ubweya m'mabatire a lithiamu-sulfure
Batire ya lithiamu-sulfure imaonedwa kuti ndi njira ya batire yokhala ndi mphamvu zambiri chifukwa cha kuchuluka kwa mphamvu zake komanso kuteteza chilengedwe. Komabe, "shuttle effect" ya polysulfides imabweretsa moyo woipa wa kuzungulira ndi kudzitulutsa kwambiri, zomwe zimalepheretsa kugwiritsa ntchito kwake.
Thiothreitol (DTT) ikhoza kuwonjezeredwa ku batri ngati chodulira. Imatha kudula ma bond a disulfide mwachangu kutentha kwa chipinda, kudula ma polysulfide apamwamba kuti apewe kusungunuka kwawo, kuletsa mphamvu ya shuttle, ndikuwonjezera mphamvu ya lithiamu ya mabatire a sulfure.
Dithiothreitol (DTT) ngati chowonjezera cha electrolyte m'mabatire a aluminiyamu/mpweya a alkaline
Mu mabatire a aluminiyamu/mpweya a alkaline, dithiothreitol imatha kupanga gawo loteteza lofanana komanso lokhazikika kudzera mu ma dynamic covalent bonds pamwamba pa anode ya aluminiyamu, kuletsa kudziwononga kwa anode ya aluminiyamu, ndikuwonjezera bwino magwiridwe ake.
Nthawi yotumizira: Disembala 31-2021