Pali ngwazi zambiri zosaimbidwa zomwe zili pafupi nafe, zomwe zimawoneka ngati zachilendo, koma kwenikweni zimatithandiza kwambiri. Proteinase K ndiye "ngwazi yosaimbidwa" mumakampani ofufuza za mamolekyu, ngakhale poyerekeza ndi "yaikulu komanso yamphamvu" mumakampaniwa, proteinase K ndi yotsika kwambiri kotero kuti takhala tikunyalanyaza kufunika kwake kwa nthawi yayitali. Ndi kufalikira kwa mliri watsopano wa korona, kufunikira kwa proteinase K kwakwera kwambiri, ndipo kupezeka kwa proteinase K kunyumba ndi kunja kuli kutali kwambiri ndi kugwiritsidwa ntchito, ndipo aliyense mwadzidzidzi anazindikira kuti proteinase K ndi yofunika kwambiri.
Kodi kugwiritsa ntchito proteinase K n'chiyani?
Proteinase K ndi serine protease yokhala ndi proteolytic enzyme ndipo imatha kugwira ntchito m'malo osiyanasiyana (pH (4-12.5), mchere wambiri, kutentha kwa 70°C, ndi zina zotero). Kuphatikiza apo, ntchito ya proteinase K siletsedwa ndi SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, ndi zina zotero, ndipo kuchuluka kwa sopo wothira kungathandizenso ntchito ya proteinase K. Mu chithandizo chamankhwala (mavairasi ndi ma microbial disinfection), chakudya (kufewetsa nyama), chikopa (kufewetsa tsitsi), kupanga vinyo (kuyeretsa mowa), kukonzekera amino acid (nthenga zoonongeka), kuchotsa nucleic acid, in situ hybridization, ndi zina zotero, proteinase K Pali ntchito. Ntchito yomwe imagwiritsidwa ntchito kwambiri ndi kuchotsa nucleic acid.
Proteinase K imatha kupanga ma enzyme amitundu yonse ya mapuloteni omwe ali mu chitsanzocho, kuphatikizapo ma histone omwe amamangiriridwa mwamphamvu ku ma nucleic acid, kotero kuti ma nucleic acid amatha kutulutsidwa kuchokera mu chitsanzocho ndikutulutsidwa mu chotsitsacho, zomwe zimathandiza gawo lotsatira lochotsa ndi kuyeretsa. Pakupeza viral nucleic acid, proteinase K ndi chimodzi mwa zinthu zofunika kwambiri mu yankho la sampuli ya kachilomboka. Proteinase K imatha kuswa ndikuletsa puloteni yophimba ya kachilomboka, yomwe ndi yotetezeka panthawi yonyamula ndi kuzindikira; kuphatikiza apo, proteinase K imathanso kuwononga RNase. Imaletsa kuwonongeka kwa RNA ya viral ndikuthandizira kuzindikira nucleic acid.
Kutchuka kwa usiku wonse kwa proteinase K
Kaya ndi kafukufuku wa sayansi kapena IVD, kuchotsa nucleic acid ndiye njira yofunikira kwambiri, kotero proteinase K yakhala yofunika kwambiri nthawi zonse. Komabe, kale, proteinase K sinali yodziwika bwino kuposa ntchito yake. Gawo lalikulu la izi linali chifukwa chakuti ubale wa proteinase K ndi kufunikira kwake unali wokhazikika. Anthu ochepa angaganize kuti proteinase K ingakhale vuto.
Pamene mliri watsopano wa korona unayamba, kufunika kwa mayeso a nucleic acid kwawonjezeka. Pofika kumapeto kwa June 2020, China yamaliza mayeso atsopano a korona pafupifupi 90 miliyoni, ndipo chiwerengerochi n'choopsa kwambiri padziko lonse lapansi. Mu mayeso ochotsa nucleic acid, kuchuluka kwa proteinase K komwe kumagwira ntchito ndi pafupifupi 50-200 μg/mL. Nthawi zambiri, pamafunika pafupifupi 100 μg ya proteinase K kuti ichotse chitsanzo cha nucleic acid. Pogwiritsidwa ntchito kwenikweni, kuti muwonjezere mphamvu yochotsera nucleic acid, nthawi zambiri Proteinase K imagwiritsidwa ntchito mochuluka. Kuzindikira nucleic acid ya coronavirus yatsopano kwabweretsa kuchuluka kwa proteinase K. Kuchuluka koyambirira kwa proteinase K kunasweka mwachangu, ndipo proteinase K inakhala chinthu chofunikira chopewera miliri usiku wonse.
Mavuto pakupanga proteinase K
Ngakhale kuti chifukwa cha kukula kwa mliriwu, kufunika kwa proteinase K kwakhala kofunikira kwa anthu, n’zochititsa manyazi kuti chifukwa cha kuchepa kwa proteinase K, makampani ochepa m’dziko muno akhala akugwira ntchito yopanga proteinase K. Anthu akafuna kukhazikitsa kupanga proteinase K. Pa nthawi yopanga, zinapezeka kuti proteinase K ndi puloteni yapadera kwambiri. N’zovuta kwambiri kukulitsa mphamvu yopanga proteinase K m’kanthawi kochepa.
Kupanga kwakukulu kwa proteinase K kumakumana ndi mavuto awa:
1. Kuchepa kwa mawu
Proteinase K imatha kuwononga mapuloteni ambiri mosafunikira kwenikweni ndikuyambitsa poizoni woopsa ku selo lodziwika bwino. Chifukwa chake, kuchuluka kwa proteinase K nthawi zambiri kumakhala kochepa kwambiri. Kuwunika machitidwe ofotokozera ndi mitundu yomwe imatulutsa proteinase K kwambiri nthawi zambiri kumafuna nthawi yayitali.
2. Zotsalira za utoto ndi ma nucleic acid
Kuphika kwakukulu kumabweretsa kuchuluka kwa utoto ndi zotsalira za nucleic acid zomwe zimapezeka mu utoto. N'zovuta kuchotsa zonyansa izi pogwiritsa ntchito njira yosavuta yoyeretsera, ndipo kuyeretsa kovuta kumawonjezera mtengo ndikuchepetsa kuchuluka kwa kuchira.
3. Kusakhazikika
Proteinase K si yokhazikika mokwanira, imatha kudzipanga yokha, ndipo zimakhala zovuta kuisunga bwino pa 37°C kwa nthawi yayitali popanda choteteza.
4. Zosavuta kuzizira
Pokonzekera ufa wouma wozizira wa proteinase K, kuti muwonetsetse kuti kuchuluka kwa proteinase K mu ufa wouma wozizira ndi kwakukulu, ndikofunikira kuwonjezera choteteza chouma chozizira pamlingo waukulu, koma kuchuluka kwa proteinase K kukafika pa 20mg/mL ndi kupitirira apo, zimakhala zosavuta. Kuphatikizika kumapanga mpweya wozizira, zomwe zimabweretsa zovuta zazikulu pakuuma kwa proteinase K ndi kuchuluka kwakukulu.
5. Ndalama zambiri
Proteinase K ili ndi protease yambiri ndipo imatha kupha ma protease ena mu labotale. Chifukwa chake, proteinase K imafuna malo apadera opangira, zida, ndi antchito ofufuza, kupanga ndi kupanga.
Yankho la XD BIOCHEM la proteinase K
XD BIOCHEM ili ndi njira yodziwika bwino yowonetsera ndi kuyeretsa mapuloteni, ndipo ili ndi chidziwitso chochuluka pakuwonetsa ndi kuyeretsa mapuloteni obwerezabwereza komanso kukonza njira zopangira. Kudzera mu kupanga mwachangu kwa gulu lofufuza ndi kupanga, njira yayikulu yopangira proteinase K yatha. Kutulutsa kwa mwezi uliwonse kwa ufa wouma wozizira kumaposa 30 KG. Chogulitsachi chili ndi magwiridwe antchito okhazikika, ntchito yayikulu ya enzyme, komanso palibe zotsalira za cytochrome ndi nucleic acid. Takulandirani kuti mulumikizane ndi XD BIOCHEM Pezani phukusi loyesera (Imelo:sales@xdbiochem.comFoni: +86 513 81163739).
Mayankho aukadaulo a XD BIOCHEM akuphatikizapo
Pogwiritsa ntchito kuphatikiza kwa plasmid yokhala ndi makope ambiri, mitundu yotulutsa mawu ambiri yokhala ndi kuchuluka kwa mawu ofotokozera a 8g/L imasankhidwa, zomwe zimathandiza kuthetsa vuto la kuchuluka kwa proteinase K komwe kumawonetsa mawu ofotokozera.
Kudzera mu njira yoyeretsera zinthu m'njira zambiri, ma cytochrome ndi nucleic acid residues a proteinase K adachotsedwa bwino pansi pa mtengo wokhazikika.
Pogwiritsa ntchito njira yowunikira bwino kwambiri ya ma buffer oteteza, buffer yomwe imatha kusunga proteinase K mosalekeza pa 37°C inasankhidwa.
Ma buffer oyezera amathetsa vuto lakuti proteinase K ndi yosavuta kuisonkhanitsa ndi kuipangitsa kukhala yolimba kwambiri, ndipo imayika maziko a proteinase K youma ndi kuizizira kwambiri.
Chitsanzo cha XD BIOCHEM proteinase K
Mayeso okhazikika a XD BIOCHEM proteinase K: sipadzakhala kusintha kwakukulu pa ntchito pambuyo pa masiku 80 kutentha kwa chipinda
Mayeso okhazikika a XD BIOCHEM proteinase K: sipadzakhala kusintha kwakukulu pa ntchito pambuyo pa masiku 80 kutentha kwa chipinda.
Kuyerekeza zotsatira za kuchotsa nucleic acid ya XD BIOCHEM proteinase K ndi zinthu zina zopikisana. Pochotsa nucleic acid, XD BIOCHEM ndi competitive proteinase K zimagwiritsidwa ntchito motsatana. Mphamvu ya kuchotsa XD BIOCHEM proteinase K ndi yayikulu ndipo Ct ya jini yofunidwa ndi yotsika.
Nthawi yotumizira: Disembala 31-2021