P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Kuzindikira zochita za beta-galactosidase: PNPG imagwiritsidwa ntchito poyesa kuyeza ntchito ya beta-galactosidase, puloteni yomwe imayambitsa hydrolysis ya lactose kukhala shuga ndi galactose.Hydrolysis ya PNPG yopangidwa ndi beta-galactosidase imatulutsa molekyulu ya p-nitrophenol (pNP), yomwe imatha kuzindikirika chifukwa cha mtundu wake wachikasu.

Kuwunika kwa ma enzyme inhibitors ndi ma activator: PNPG itha kugwiritsidwa ntchito powunikira kwambiri kuti muzindikire zinthu zomwe zimathandizira ntchito ya beta-galactosidase.Poyesa kuchuluka kwa PNPG hydrolysis pamaso pa mitundu yosiyanasiyana yoyesera, ofufuza amatha kuzindikira zoletsa zomwe zimachepetsa ntchito ya ma enzyme kapena zoyambitsa zomwe zimathandizira ma enzyme.

Kuphunzira kwa enzyme kinetics: The hydrolysis ya PNPG yopangidwa ndi beta-galactosidase imatsatira Michaelis-Menten kinetics, kulola ofufuza kudziwa magawo ofunikira a enzyme monga kuthamanga kwambiri (Vmax) ndi Michaelis constant (Km).Chidziwitsochi chimathandizira kumvetsetsa kuyanjana kwa gawo lapansi la enzyme komanso kuchita bwino kwambiri.

Kugwiritsa ntchito kwa mamolekyulu a biology: Beta-galactosidase, yomwe imadula PNPG, imagwiritsidwa ntchito ngati jini ya mtolankhani mu biology ya maselo.Gawo laling'ono la PNPG nthawi zambiri limagwiritsidwa ntchito kuti lizindikire ndikuwona mawonekedwe a mtolankhani, ndikupereka njira yosavuta komanso yovuta yowunika mawonekedwe a jini m'machitidwe osiyanasiyana oyesera.