Phenylgalactoside CAS:2818-58-8

Zotsatira pa ntchito ya enzyme: Phenylgalactoside imagwiritsidwa ntchito kwambiri poyesa ntchito ya enzyme ya β-galactosidase. Phenylgalactoside ikasungunuka ndi β-galactosidase, imatulutsa p-nitrophenol. Kuchuluka kwa p-nitrophenol kumatha kuyezedwa mochuluka, kupereka chidziwitso pa ntchito ya β-galactosidase. Zotsatirazi zimagwiritsidwa ntchito poyesa ma enzyme ndi machitidwe owunikira.

Kusanthula kwa majini: Phenylgalactoside nthawi zambiri imagwiritsidwa ntchito ngati gawo la kafukufuku wa biology ya mamolekyulu kuti aphunzire za kufotokozera kwa majini. Jini la lacZ, lomwe limapanga β-galactosidase, nthawi zambiri limaphatikizidwa ndi malamulo oyendetsera majini ena ofunikira. Kufotokozera kwa jini la lacZ ndi hydrolysis ya phenylgalactoside ndi β-galactosidase zitha kusonyeza mawonekedwe ndi mulingo wa jini lomwe likuphunziridwa.

Machitidwe owunikira: Phenylgalactoside ndi gawo lofunika kwambiri la machitidwe owunikira omwe amagwiritsa ntchito ntchito ya β-galactosidase. Chitsanzo chimodzi chodziwika bwino ndi njira yowunikira yabuluu-yoyera, yomwe imagwiritsidwa ntchito kuzindikira maselo obwerezabwereza kapena osinthika mu zoyeserera za biology ya mamolekyu. Makoloni omwe atenga bwino DNA yobwerezabwereza kapena omwe adakumana ndi majini obwerezabwereza adzawonetsa β-galactosidase, zomwe zimapangitsa kuti phenylgalactoside iwonongeke komanso kuti mtundu wabuluu upangidwe.

Kuyeretsa mapuloteni: Nthawi zina, phenylgalactoside ingagwiritsidwe ntchito ngati ligand ya affinity chromatography kuti itsuke mapuloteni omwe amamangirira kapena kuyatsidwa ndi β-galactosidase. Puloteni yomwe imakonda ikhoza kukhala ndi affinity tag kapena fusion tag yokhala ndi β-galactosidase-binding domain. Mwa kudutsa protein mix kudzera mu column yokhala ndi phenylgalactoside yosayenda, protein yomwe mukufunayo ikhoza kusungidwa mosankha kenako nkutha.