PIPES mchere wa monosodium CAS: 10010-67-0

Buffering Agent: HEPES-Na makamaka amagwiritsidwa ntchito ngati chotchinga chosungira kuti asunge pH yokhazikika pakuyesa kwachilengedwe ndi zamankhwala.Itha kukana bwino kusintha kwa pH komwe kumachitika chifukwa chowonjezera ma acid kapena maziko.

Chikhalidwe Cha Ma cell: HEPES-Na nthawi zambiri imawonjezeredwa ku media media media kuti ipereke malo okhazikika komanso abwino kwambiri a pH kuti ma cell akule komanso kuti azitha kuchita bwino.Zimathandiza kuthana ndi kusinthasintha kwa pH komwe kungachitike chifukwa cha kagayidwe kachakudya ka maselo amoyo.

Kuyeza kwa Enzyme: HEPES-Na imagwiritsidwa ntchito ngati buffer pakuyesa ma enzyme.Zimathandizira kuti pH ikhale pamlingo woyenera kwambiri pakuchita kwa ma enzyme ndi kukhazikika, kuwonetsetsa kuti zotsatira zolondola komanso zodalirika.

Njira za Biology ya Molecular: HEPES-Na imagwiritsidwa ntchito kwambiri munjira zosiyanasiyana zamamolekyulu, monga kudzipatula kwa DNA ndi RNA, kukulitsa kwa PCR, ndi kusanthula mapuloteni.Zimathandizira kuti pH ikhale yokhazikika panthawiyi, zomwe ndizofunikira kwambiri kuti ma molekyulu achilengedwe azikhala osakhulupirika.

Electrophoresis: Mu gel electrophoresis, HEPES-Na imagwiritsidwa ntchito ngati chotchinga kuti chipereke malo okhazikika a pH olekanitsa DNA, RNA, ndi mapuloteni.Zimathandiza kuonetsetsa kusamuka koyenera ndi kusamuka kwa mamolekyu mkati mwa matrix a gel.