5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ndi gulu lomwe limagwiritsidwa ntchito m'maphunziro osiyanasiyana a biochemical, makamaka pozindikira ndikuwona zochitika za enzyme.Ndi gawo lapansi lomwe limatha kupangidwa ndi hydrolyzed ndi michere inayake, zomwe zimapangitsa kutulutsa kwamtundu wamtundu kapena fulorosenti.
Pagululi nthawi zambiri amagwiritsidwa ntchito poyesa kudziwa kukhalapo ndi zochita za michere monga beta-galactosidase ndi beta-glucuronidase.Ma enzymes awa amadula magulu a acetyl ndi glucosaminide kuchokera ku gawo lapansi, zomwe zimapangitsa kupanga chromophore ya buluu kapena yobiriwira.
Mapangidwe apadera a 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide amalola kuti azindikire mosavuta ndi kuchuluka kwa ntchito ya enzyme.Kugwiritsiridwa ntchito kwake mu njira zosiyanasiyana zoyesera, kuphatikizapo histochemistry, immunohistochemistry, ndi ma cell-based assays, zathandizira kumvetsetsa bwino ntchito za enzyme.