Proteinase K CAS:39450-01-6 Mtengo wa Wopanga

Kugaya ndi Kuyeretsa Mapuloteni: Proteinase K imagwiritsidwa ntchito nthawi zambiri poyeretsa mapuloteni kuti achotse mapuloteni osafunikira kapena zinthu zina zodetsa. Imatha kugawa bwino ma peptide bonds ndikugaya mapuloteni, zomwe zimathandiza kuti mapuloteni omwe akufuna azitha kugayidwa komanso kutsukidwa.

Kutulutsa Nucleic Acid: Proteinase K imagwiritsidwa ntchito nthawi zonse mu njira zotulutsira DNA ndi RNA. Mwa kuswa mapuloteni ndikuchotsa ma nuclease, zimathandiza kulekanitsa DNA kapena RNA yapamwamba popanda kuwonongeka. Proteinase K imatha kuthana ndi mitundu yosiyanasiyana ya zitsanzo, kuphatikizapo magazi, minofu, kapena maselo.

Kuyeretsa kwa PCR: Proteinase K imathandizanso poyeretsa PCR. Imatha kuchotsa ma enzyme osafunikira, ma primer, ma nucleotide, kapena zinthu zina zodetsa zomwe zingasokoneze ntchito zoyambira. Kugaya kwa Proteinase K kumathandiza kupeza zinthu zoyera za PCR zoyenera kufufuzidwa kwina.

Kujambula Zala za DNA: Pojambula zala za DNA kapena kusanthula kwa forensic, Proteinase K imagwiritsidwa ntchito kuchotsa DNA kuchokera ku zitsanzo zosiyanasiyana zamoyo, monga magazi, malovu, kapena tsitsi. Kutha kwake kuwononga mapuloteni kumathandiza kuswa nembanemba ya maselo, kutulutsa DNA kuti iwunikidwenso pambuyo pake.

Histology ndi Immunohistochemistry: Proteinase K imathandiza kupeza ma antigen mu njira za histology ndi immunohistochemistry. Imatha kuswa maulalo olumikizana omwe amapangidwa panthawi yokonza zitsanzo, zomwe zimathandiza kuti ma antigen azitha kupezeka bwino komanso kukonza mtundu wa utoto m'magawo a minofu.

Kuletsa Enzyme: Proteinase K nthawi zambiri imagwiritsidwa ntchito kuletsa ma enzyme. Mphamvu yake yowononga ma enzyme imaletsa kuipitsidwa komwe kumachitika chifukwa cha zomwe zachitika kale, zomwe zimapangitsa kuti ikhale chida chofunikira kwambiri mu kafukufuku wambiri wa sayansi ya zamoyo.

Kapangidwe ka Mapuloteni ndi Kuphunzira Ntchito Zake: Proteinase K imagwiritsidwa ntchito mu kafukufuku kuti iphunzire kapangidwe ka mapuloteni ndi ntchito zake. Mwa kugawa ma peptide bonds m'malo enaake, ingathandize kuzindikira madera ofunikira kapena madera ogwira ntchito mkati mwa mapuloteni..