SYBR GREEN I Nucleic Acid Gel Stain CAS:163795-75-3

SYBR Green I ndi utoto wa gel wa nucleic acid womwe umagwiritsidwa ntchito kwambiri mu kafukufuku wa biology ya molecular. Ndi utoto wa fluorescent womwe umalumikizana mosankha ndi mamolekyu a DNA ndi RNA okhala ndi zingwe ziwiri, zomwe zimapangitsa kuti ziwonekere pansi pa kuwala kwa ultraviolet. SYBR Green I ndi yothandiza kwambiri ndipo ingagwiritsidwe ntchito kuzindikira ndikuwerengera DNA kapena RNA mu agarose kapena polyacrylamide gels. Imagwiritsidwa ntchito kwambiri pa electrophoresis ya gel ya DNA, kusanthula kwa PCR, ndi ntchito zina za biology ya molecular.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

DNA gel electrophoresis: SYBR Green I stain imawonjezeredwa ku DNA gel pambuyo pa electrophoresis, ndipo ikayikidwa pa kuwala kwa UV, imalumikizana ndi mikanda ya DNA, ndikupanga kuwala kowala. Njira iyi yopaka utoto imathandiza kuwona ndikuwunika zidutswa za DNA zomwe zalekanitsidwa ndi kukula kwake.

Kuchuluka kwa PCR (qPCR): SYBR Green I imagwiritsidwa ntchito kwambiri mu qPCR kuyang'anira kukulitsa kwa DNA kapena RNA nthawi yeniyeni. Utotowo umalumikizana ndi DNA yokhala ndi zingwe ziwiri yomwe imapangidwa panthawi ya kukulitsa kwa PCR, ndipo kuwonjezeka kwa mphamvu ya fluorescence kumayesedwa pa nthawi iliyonse yokulitsa, zomwe zimapangitsa kuti chiwerengero cha template ya nucleic acid yoyambira chiwerengedwe.

RNA gel electrophoresis: SYBR Green I ingagwiritsidwenso ntchito pa RNA gel electrophoresis, zomwe zimathandiza kuwona ndi kusanthula zidutswa za RNA. Ndi yothandiza pakuwunika umphumphu wa RNA komanso pophunzira kuchuluka kwa RNA.

Kulemba ndi kuzindikira ma nucleic acid: SYBR Green I ingagwiritsidwe ntchito kulemba ma nucleic acid ndi fluorescence. Izi zitha kuchitika pophatikiza utoto panthawi yopanga ma nucleic acid kapena pogwiritsa ntchito njira zolembera pambuyo pake. Ma nucleic acid omwe ali ndi ma label amatha kuwonetsedwa ndikupezeka pogwiritsa ntchito njira zojambulira za fluorescent.

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C32H37N4S
Kuyesa	99%
Maonekedwe	Madzi opanda mtundu
Nambala ya CAS	163795-75-3
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.