Tris(hydroxymethyl)nitromethane CAS:126-11-4

Zotsatira:

Kutha Kusunga Zinthu Zofunikira: Tris imagwira ntchito ngati chothandizira chosunga zinthu zopindulitsa chifukwa cha kuthekera kwake kulandira kapena kupereka ma proton, kusunga pH yokhazikika mu mayankho. Nthawi zambiri imagwiritsidwa ntchito ngati gawo lalikulu mu machitidwe osunga zinthu zopindulitsa kuti ikhazikitse pH ya zitsanzo zamoyo ndi zochita zake.

Mapulogalamu:

Biology ya Mamolekyulu: Tris imagwiritsidwa ntchito kwambiri ngati chotetezera mu njira zosiyanasiyana za biology ya mamolekyulu, kuphatikizapo DNA ndi RNA isolation, PCR, gel electrophoresis, ndi protein purification. Imathandiza kusunga pH yokhazikika, zomwe zimathandiza kuti zinthu zikhale bwino kwambiri pakuchita zinthu za enzymatic komanso kuyanjana kwa mamolekyulu.

Chikhalidwe cha Maselo: Tris nthawi zambiri imagwiritsidwa ntchito muzomera za maselo kuti ikhale ndi pH yokhazikika komanso osmotic balance, kuthandizira kukula kwa maselo abwino komanso kukhala ndi moyo wabwino.

Chemistry ya Mapuloteni: Tris imagwiritsidwa ntchito poyesa chemistry ya mapuloteni, monga kusungunuka kwa mapuloteni, kuyesa kukhazikika kwa mapuloteni, ndi maphunziro omanga mapuloteni ndi ligand. Zimathandiza kusunga pH yofunikira, kuonetsetsa kuti mapuloteni akupindika bwino komanso akugwira ntchito moyenera.

Kapangidwe ka Enzymology: Tris imagwiritsidwa ntchito poyesa ma enzymatic osiyanasiyana kuti ikwaniritse bwino momwe pH imagwirira ntchito. Imatsimikizira zotsatira zodalirika komanso zobwerezabwereza, zomwe zimathandiza kuyeza molondola kayendedwe ka enzyme ndi maphunziro oletsa.

Kuyesa kwa Biochemical: Tris imagwiritsidwa ntchito ngati gawo la mayeso ambiri a biochemical chifukwa cha mphamvu zake zotetezera. Imasunga pH yosasintha panthawi yoyesa colorimetric, spectrophotometric, ndi enzymatic, zomwe zimapangitsa kuti zotsatira zake zikhale zolondola komanso zodalirika.