3-(cyclohexylamino) -2-hydroxy-1-propanesuhicic acid CAS:73463-39-5

CAPSO (3-(cyclohexylamino) -2-hydroxypropanesulfonic acid) ndi zwitterionic buffer yomwe imagwiritsidwa ntchito kwambiri mu biochemistry ndi molecular biology.Imadziwika ndi mphamvu yake yotchinga kwambiri pa pH yotakata ndipo imatengedwa kuti ndi yabwino m'malo mwa ma buffer ena monga MOPS ndi MES.

Chotsatira chachikulu cha CAPSO ndi kuthekera kwake kosunga malo okhazikika a pH pakuyesa kwachilengedwe.Zimagwira ntchito popereka kapena kuvomereza ma protoni kuti muchepetse kusintha kwa pH komwe kumachitika chifukwa cha ma acid kapena maziko owonjezera.Mtengo wake wa pKa uli pafupi ndi 9.8, zomwe zimapangitsa kukhala chitetezo chothandiza pakuyesa mu pH ya 8.2-9.6.

CAPSO imagwiritsidwa ntchito nthawi zambiri pazinthu monga kuyeretsa mapuloteni, kuyesa kwa ma enzyme, ndi electrophoresis.Kukhazikika kwake komanso kusokoneza pang'ono kwachilengedwe kumapangitsa kuti ikhale yothandiza kukhalabe ndi mikhalidwe yabwino kwambiri yazachilengedwe.Kuphatikiza apo, CAPSO imagwiritsidwa ntchito nthawi zambiri powerenga mawonekedwe a protein, kupindika kwa mapuloteni, komanso kukhazikika.