3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2
Kuzindikira zochitika za beta-galactosidase: ONPG imagwiritsidwa ntchito nthawi zambiri kudziwa kukhalapo ndi zochitika za beta-galactosidase mu zitsanzo zosiyanasiyana zamoyo, monga zikhalidwe za bakiteriya kapena ma cell lysates.Kupanga kwa o-nitrophenol, komwe kuli ndi mtundu wachikasu, kungayesedwe mosavuta pogwiritsa ntchito spectrophotometer.
Maphunziro a ma gene: ONPG imagwiritsidwa ntchito kwambiri pakufufuza kwa ma cell a biology kuti aphunzire za jini.Pophatikiza wolimbikitsa jini yosangalatsa ndi jini yosunga beta-galactosidase, ofufuza atha kuyeza zomwe wotsatsayu achita powonjezera ONPG ndikuwerengera zotsatira za o-nitrophenol.Njirayi, yomwe imadziwika kuti beta-galactosidase reporter assay, imapereka chidziwitso chokhudza zolemba za jini.
Chizindikiritso cha mabakiteriya: Mabakiteriya ena amapanga beta-galactosidase, pamene ena samatero.ONPG itha kugwiritsidwa ntchito limodzi ndi mayeso ena a biochemical kuti azindikire mitundu ya mabakiteriya potengera kuthekera kwawo kwa ONPG hydrolyze.Njirayi imagwiritsidwa ntchito kwambiri pofufuza zachipatala ndi ma laboratories a microbiology.
Kuwunika kwa ma enzyme inhibitors kapena activator: ONPG itha kugwiritsidwa ntchito kuwunikira zinthu zomwe zimathandizira ntchito ya beta-galactosidase.Poyesa ntchito ya ma enzyme pamaso pa mankhwala osiyanasiyana, ochita kafukufuku amatha kuzindikira zoletsa kapena zoyambitsa zomwe zitha kufufuzidwanso chifukwa chakuchiritsa kwawo.
Kupanga | C12H15NO8 |
Kuyesa | 99% |
Maonekedwe | Choyeraufa |
CAS No. | 3150-25-2 |
Kulongedza | Zing'onozing'ono ndi zambiri |
Shelf Life | zaka 2 |
Kusungirako | Sungani pamalo ozizira ndi owuma |
Chitsimikizo | ISO. |