4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Mphamvu ya 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) ndi yogwira ntchito ngati gawo la enzyme beta-glucosidase. Enzyme iyi imadula mgwirizano wa glucosidi wa MUG, zomwe zimapangitsa kuti 4-Methylumbelliferone (4-MU) itulutsidwe. Kugwiritsa ntchito MUG makamaka kumachitika m'munda wa tizilombo toyambitsa matenda, makamaka pozindikira ndi kuzindikira mabakiteriya opanga beta-glucosidase. MUG imagwiritsidwa ntchito kwambiri pozindikira Escherichia coli (E. coli) m'madzi ndi m'zakudya. E. coli ili ndi enzyme beta-glucosidase, yomwe imatha kupha MUG ndikupereka chizindikiro cha fluorescent pamaso pa kuwala kwa ultraviolet (UV). Mphamvu ya fluorescent ya 4-MU imalola kuti zidziwike mosavuta komanso kuwerengera. Pamene gawo la MUG laphatikizika ndi hydrolyzed, 4-MU yopangidwa imatulutsa kuwala kwabuluu, zomwe zimathandiza kuzindikira mabakiteriya omwe ali ndi ntchito ya beta-glucosidase. Njirayi imagwiritsidwa ntchito kwambiri poyang'anira chilengedwe komanso kuyesa chitetezo cha chakudya, chifukwa imapereka njira yofulumira komanso yodziwira kuipitsidwa kwa mabakiteriya. Kuphatikiza pa kugwiritsidwa ntchito kwake mu microbiology, MUG ingagwiritsidwenso ntchito kuphunzira ntchito ndi kuletsa kwa beta-glucosidase mu kafukufuku wa biochemistry ndi molecular biology. Kuwala kwake kumathandiza kuyeza kinetics ya enzyme ndipo kungagwiritsidwe ntchito pofufuza zoletsa kapena zoyambitsa ntchito ya beta-glucosidase. Ponseponse, MUG ndi mankhwala osinthika omwe amagwiritsidwa ntchito kwambiri m'magawo a microbiology, biochemistry, ndi molecular biology pozindikira ntchito ya beta-glucosidase komanso kuzindikira mabakiteriya omwe amapanga enzyme iyi.