4-Nitrophenyl Beta-D-glucuronide CAS: 10344-94-2

4-Nitrophenyl Beta-D-glucuronide ndi mankhwala omwe amapangidwa polumikiza molekyulu ya shuga ku gulu la 4-nitrophenyl kudzera mu kulumikizana kwa glycosidic. Kawirikawiri imagwiritsidwa ntchito ngati gawo la mayeso a enzymatic kuti izindikire kukhalapo ndi ntchito ya β-glucuronidase, enzyme yomwe imakhudzidwa ndi kagayidwe ka mankhwala osiyanasiyana ndi ma xenobiotics m'zinyama zoyamwitsa. Pamene β-glucuronidase ilipo, imadula mgwirizano wa glycosidic pakati pa shuga ndi gulu la 4-nitrophenyl, zomwe zimapangitsa kuti 4-nitrophenol itulutsidwe, yomwe ingapezeke mosavuta pa 400-420 nm. Kachitidwe ka enzymatic aka kamapereka muyeso wochuluka wa ntchito ya β-glucuronidase ndipo nthawi zambiri imagwiritsidwa ntchito ngati chida chopezera mankhwala, maphunziro a poizoni, komanso matenda azachipatala.

Oda kapena Pemphani Mtengo

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Kugwiritsa Ntchito ndi Zotsatira zake

Kuzindikira ntchito ya β-glucuronidase: 4-NPBG imagwiritsidwa ntchito kwambiri ngati gawo la chromogenic kuti liwone kupezeka ndi ntchito ya β-glucuronidase m'masampulu osiyanasiyana achilengedwe. Enzymeyi imadula mgwirizano wa glycosidic wa 4-NPBG, ndikupanga 4-nitrophenol, yomwe imatha kuyezedwa pogwiritsa ntchito spectrophotometry.

Maphunziro a kagayidwe ka mankhwala: Popeza β-glucuronidase imagwira ntchito yofunika kwambiri pa kagayidwe ka mankhwala ndi ma xenobiotics, 4-NPBG ingagwiritsidwe ntchito poyesa ntchito ya enzyme iyi mu maphunziro a kagayidwe ka mankhwala. Zimathandiza kumvetsetsa kukula ndi kayendedwe ka zochita za glucuronidation, zomwe ndizofunikira pakuchotsa mankhwala ndi kupezeka kwa bioavailability.

Maphunziro a Zauchidakwa: Mankhwala ena a poizoni amatha kusinthidwa kukhala glucuronide conjugates. Pogwiritsa ntchito 4-NPBG ngati substrate, ofufuza amatha kufufuza momwe β-glucuronidase imagwirira ntchito m'maselo osiyanasiyana kapena m'mizere ya maselo kuti awone ngati mankhwalawo ali ndi poizoni kapena zotsatirapo zoyipa.

Kuzindikira matenda: Kuyeza kwa ntchito ya β-glucuronidase pogwiritsa ntchito 4-NPBG kungagwiritsidwe ntchito m'malo azachipatala kuti mudziwe matenda kapena mikhalidwe ina. Kuchuluka kosazolowereka kapena ntchito ya β-glucuronidase kungasonyeze matenda enaake a majini, kulephera kugwira ntchito bwino kwa chiwindi, kapena khansa.

Chitsanzo cha Zamalonda

Kulongedza Zinthu:

Zina Zowonjezera:

Kapangidwe kake	C12H13NO9
Kuyesa	99%
Maonekedwe	Ufa woyera
Nambala ya CAS	10344-94-2
Kulongedza	Kakang'ono komanso kochuluka
Moyo wa Shelufu	zaka 2
Malo Osungirako	Sungani pamalo ozizira komanso ouma
Chitsimikizo	ISO.