4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Zotsatira: ONPG ndi gawo lapansi lomwe limagwiritsidwa ntchito kuzindikira kupezeka ndi zochitika za enzyme β-galactosidase.Pamene β-galactosidase enzyme ilipo ndipo ikugwira ntchito, imadula ONPG kukhala zinthu ziwiri: o-nitrophenol ndi chochokera ku galactose.Kumasulidwa kwa o-nitrophenol kumabweretsa kusintha kwa mtundu wachikasu, komwe kungathe kuyeza pogwiritsa ntchito spectrophotometer.

Kugwiritsa ntchito: ONPG ili ndi ntchito zingapo mu biology ya maselo ndi kafukufuku wa biochemistry:

Kutsimikiza kwa ntchito ya β-galactosidase: ONPG imagwiritsidwa ntchito nthawi zambiri kuyeza ndi kuwerengera ntchito ya β-galactosidase enzyme.Mlingo wa mapangidwe a o-nitrophenol, womwe umagwirizana mwachindunji ndi ntchito ya enzyme, ukhoza kuyeza spectrophotometrically.

Kufotokozera kwa ma gene ndi kuwongolera: ONPG imagwiritsidwa ntchito nthawi zambiri pazoyeserera zokhudzana ndi mafotokozedwe amtundu ndi maphunziro owongolera.Amagwiritsidwa ntchito pafupipafupi pakuyesa kwa mapuloteni ophatikizika, monga njira yophatikizira ya lacZ, kuti aphunzire mafotokozedwe a majini motsogozedwa ndi olimbikitsa enieni.Ntchito ya beta-galactosidase yoyezedwa pogwiritsa ntchito ONPG imapereka chidziwitso pamlingo wa jini.

Kuwunika kwa ntchito ya β-galactosidase: ONPG itha kugwiritsidwa ntchito ngati njira yowunikira ma colorimetric muukadaulo wophatikizanso wa DNA kuzindikira kukhalapo ndi magwiridwe antchito a jini ya LacZ, yomwe imasunga β-galactosidase.Njira yowunikirayi imathandizira kuzindikira ma clones omwe ali ndi jini yosangalatsa.

Maphunziro a enzyme kinetics: ONPG ndiyothandizanso powerenga kinetics ya β-galactosidase enzyme.Poyesa kuchuluka kwa ma enzyme-substrate reaction pamagulu osiyanasiyana a gawo lapansi, ndizotheka kudziwa magawo a kinetic monga Michaelis-Menten constants (Km) ndi kuchuluka kwamachitidwe (Vmax).